Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test28settembre
From 2010.igem.org
m (→Methods) |
m (→Methods) |
||
Line 34: | Line 34: | ||
Optical density (O.D.) of each cell culture was than measured with TECAN Infinte F200. Samples were diluted in order to obtain the same O.D. equal to 0,02. | Optical density (O.D.) of each cell culture was than measured with TECAN Infinte F200. Samples were diluted in order to obtain the same O.D. equal to 0,02. | ||
- | Than we performed a 21 hours' experiment with measurements of absorbance and green fluorescence every five minutes with TECAN Infinite F200. I63 and I64 circuits were also induced 100nM with HSL directly into multiplate well. Each value shown is the mean of three measurements, from GFP data | + | Than we performed a 21 hours' experiment with measurements of absorbance and green fluorescence every five minutes with TECAN Infinite F200. I63 and I64 circuits were also induced 100nM with HSL directly into multiplate well. Each value shown is the mean of three measurements, from GFP data that of a non-fluorescent culture (negative control) was subtracted; cultures were shaked for 15 seconds every five minutes. |
==RESULTS== | ==RESULTS== |
Revision as of 22:50, 14 October 2010
|
|||||||||||||||||
|
|
|