Team:UCL London/Digestion

From 2010.igem.org

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===Method===
===Method===
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1. Add all the contents listed above to labelled eppendorfs.
1. Add all the contents listed above to labelled eppendorfs.

Revision as of 22:02, 14 October 2010

UCL IGEM 2010

RETURN TO IGEM 2010

Restriction Enzyme Digestion

Materials

Plasmid DNA: 10.5 µL/5 µL

Restriction Enzymes (depend on the requirements of the parts)

  EcoRI: 1 µL
  PstI: 1 µL
  SpeI: 1 µL
  XbaI: 1 µL
  10× NE buffer: 1.5 µL/1 µL (lid)
  BSA: 1 µL (lid)

Method

UCL-DIGE.JPG
 

1. Add all the contents listed above to labelled eppendorfs.

2. Spin for 30 sec.

3. Incubation at 37°C for 1hr.

4. Leave on ice before the gel electrophoresis


Retrieved from "http://2010.igem.org/Team:UCL_London/Digestion"