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Team:Yale/LabNotebook/Week1
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(New page: Monday 6/7--defended project (yay!) Wednesday 6/9--started in lab--got set up & started cultures of DH5alpha and LE392 Thursday 6/10--inoculated liquid cultures in AM, made up different ...)
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Revision as of 10:39, 18 June 2010
Monday 6/7--defended project (yay!)
Wednesday 6/9--started in lab--got set up & started cultures of DH5alpha and LE392
Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran first copper growth assay in plate reader in PM w/three different ODs of each strain(4 hr. protocol)
Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--b/c allowed to overgrow?
Monday 6/14--Primer design, redo of Friday's growth assay
Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived
Wednesday 6/16--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions
Thursday 6/17--more minimal media work and meeting, started BL21 culture
