Team:Stockholm/8 October 2010
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===Growth curve assay=== | ===Growth curve assay=== | ||
- | Inoculated fresh LB with 0.3 mM IPTG with ON cultures to a startup OD of 0.2 and a total volume of 50 ml. | + | Inoculated fresh LB with 0.3 mM IPTG with ON cultures to a startup OD of 0.2 and a total volume of 50 ml in 150 ml side-arm E-flasks. |
- | Experiment canceled after about an hour; will be repeated next week. | + | Was advised to use a smaller cultures for the flask size. Experiment canceled after about an hour; will be repeated next week. |
Revision as of 11:41, 14 October 2010
Andreas
Growth curve assay
Inoculated fresh LB with 0.3 mM IPTG with ON cultures to a startup OD of 0.2 and a total volume of 50 ml in 150 ml side-arm E-flasks.
Was advised to use a smaller cultures for the flask size. Experiment canceled after about an hour; will be repeated next week.