Team:Stockholm/8 October 2010

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(Andreas)
(Andreas)
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===Growth curve assay===
===Growth curve assay===
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Inoculated fresh LB with 0.3 mM IPTG with ON cultures to a startup OD of 0.2 and a total volume of 50 ml.
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Inoculated fresh LB with 0.3 mM IPTG with ON cultures to a startup OD of 0.2 and a total volume of 50 ml in 150 ml side-arm E-flasks.
-
Experiment canceled after about an hour; will be repeated next week.
+
Was advised to use a smaller cultures for the flask size. Experiment canceled after about an hour; will be repeated next week.

Revision as of 11:41, 14 October 2010


Andreas

Growth curve assay

Inoculated fresh LB with 0.3 mM IPTG with ON cultures to a startup OD of 0.2 and a total volume of 50 ml in 150 ml side-arm E-flasks.

Was advised to use a smaller cultures for the flask size. Experiment canceled after about an hour; will be repeated next week.