Team:ETHZ Basel/Biology/Implementation

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(generation of fusion proteins)
(generation of fusion proteins)
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== generation of fusion proteins ==
== generation of fusion proteins ==
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[[Image:Constructs_flowchart.jpg‎|thumb|400px|'''Schematical overview of the planned constructs.''' xxx.]]
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[[Image:Constructs_flowchart_new.jpg|thumb|400px|'''Schematical overview of the planned constructs.''' xxx.]]
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The image shows all the constructs we plan to clone. Once the BioBricks in the storage vectors are available, the fusion proteins in the working vector 1 or 2 will be generated according to the cloning strategy BBF RFC 28.
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The image shows all the fusion proteins that were planned. The BioBricks for all these constructs were generated via PCR or synthesized at GeneArt and ligated into the storage vector for sequencing. Easy cutting and pasting into working vectors should be possible with the cloning strategy BBF RFC 28.
== Modeller's input ==
== Modeller's input ==
Due to the high amount of fusion proteins that were in planing, priorities had to be distributed to the different genes. This was possible due to the various models the dry-lab team implemented.
Due to the high amount of fusion proteins that were in planing, priorities had to be distributed to the different genes. This was possible due to the various models the dry-lab team implemented.

Revision as of 14:38, 13 October 2010

Experimental results

generation of fusion proteins

Schematical overview of the planned constructs. xxx.

The image shows all the fusion proteins that were planned. The BioBricks for all these constructs were generated via PCR or synthesized at GeneArt and ligated into the storage vector for sequencing. Easy cutting and pasting into working vectors should be possible with the cloning strategy BBF RFC 28.

Modeller's input

Due to the high amount of fusion proteins that were in planing, priorities had to be distributed to the different genes. This was possible due to the various models the dry-lab team implemented.