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Team:KAIST-Korea/Project/Methods/PCR Result
From 2010.igem.org
(Difference between revisions)
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<br> | <br> | ||
<br> | <br> | ||
| + | |||
| + | == 9/17 PCR result == | ||
| + | |||
| + | <span style=font-size:12px><b>PCR composition </b></span> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td><b>Materials</b></td><td><b>Amount</b></td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Template</td><td>100pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer A</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer B</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td> | ||
| + | </tr> | ||
| + | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Total</td><td>50ul</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 1</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4 | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp25.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 2</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp26.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | == 9/17 PCR result == | ||
| + | |||
| + | <span style=font-size:12px><b>PCR composition </b></span> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td><b>Materials</b></td><td><b>Amount</b></td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Template</td><td>100pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer A</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer B</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td> | ||
| + | </tr> | ||
| + | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Total</td><td>50ul</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 1</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4 | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp25.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 2</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp26.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | |||
| + | == 9/17 PCR result == | ||
| + | |||
| + | <span style=font-size:12px><b>PCR composition </b></span> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td><b>Materials</b></td><td><b>Amount</b></td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Template</td><td>100pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer A</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer B</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td> | ||
| + | </tr> | ||
| + | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Total</td><td>50ul</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 1</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4 | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp25.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 2</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp26.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | |||
| + | == 9/17 PCR result == | ||
| + | |||
| + | <span style=font-size:12px><b>PCR composition </b></span> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td><b>Materials</b></td><td><b>Amount</b></td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Template</td><td>100pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer A</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer B</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td> | ||
| + | </tr> | ||
| + | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Total</td><td>50ul</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 1</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4 | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp25.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 2</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp26.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | |||
| + | == 9/17 PCR result == | ||
| + | |||
| + | <span style=font-size:12px><b>PCR composition </b></span> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td><b>Materials</b></td><td><b>Amount</b></td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Template</td><td>100pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer A</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer B</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td> | ||
| + | </tr> | ||
| + | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Total</td><td>50ul</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 1</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4 | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp25.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 2</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp26.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | |||
| + | == 9/17 PCR result == | ||
| + | |||
| + | <span style=font-size:12px><b>PCR composition </b></span> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td><b>Materials</b></td><td><b>Amount</b></td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Template</td><td>100pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer A</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer B</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td> | ||
| + | </tr> | ||
| + | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Total</td><td>50ul</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 1</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4 | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp25.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 2</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp26.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | |||
| + | == 9/17 PCR result == | ||
| + | |||
| + | <span style=font-size:12px><b>PCR composition </b></span> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td><b>Materials</b></td><td><b>Amount</b></td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Template</td><td>100pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer A</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer B</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td> | ||
| + | </tr> | ||
| + | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Total</td><td>50ul</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 1</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4 | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp25.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 2</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp26.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | |||
| + | == 9/17 PCR result == | ||
| + | |||
| + | <span style=font-size:12px><b>PCR composition </b></span> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td><b>Materials</b></td><td><b>Amount</b></td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Template</td><td>100pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer A</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer B</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td> | ||
| + | </tr> | ||
| + | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Total</td><td>50ul</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 1</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4 | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp25.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 2</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp26.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | |||
| + | == 9/17 PCR result == | ||
| + | |||
| + | <span style=font-size:12px><b>PCR composition </b></span> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td><b>Materials</b></td><td><b>Amount</b></td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Template</td><td>100pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer A</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer B</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td> | ||
| + | </tr> | ||
| + | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Total</td><td>50ul</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 1</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4 | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp25.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 2</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp26.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | |||
| + | == 9/17 PCR result == | ||
| + | |||
| + | <span style=font-size:12px><b>PCR composition </b></span> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td><b>Materials</b></td><td><b>Amount</b></td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Template</td><td>100pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer A</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Primer B</td><td>50pmole</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td> | ||
| + | </tr> | ||
| + | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>Total</td><td>50ul</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 1</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4 | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp25.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Exp 2</b> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table border=1> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | [[Image:KAIST-exp26.png|360px]] | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
== 9/17 PCR result == | == 9/17 PCR result == | ||
Revision as of 03:26, 17 October 2010
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 PCR Experiment
9/6 ~ 9/9 PCR ResultPCR composition
9/10 ~ 9/12 PCR ResultPCR composition
9/13 ~ 9/16 PCR ResultPCR composition
9/17 PCR resultPCR composition
9/17 PCR resultPCR composition
9/17 PCR resultPCR composition
9/17 PCR resultPCR composition
9/17 PCR resultPCR composition
9/17 PCR resultPCR composition
9/17 PCR resultPCR composition
9/17 PCR resultPCR composition
9/17 PCR resultPCR composition
9/17 PCR resultPCR composition
9/17 PCR resultPCR composition
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