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Team:Harvard/allergy/parts
From 2010.igem.org
(Difference between revisions)
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr> | <tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr> | ||
| - | <tr><td>AP1</td><td>1 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> primer1</a> </td></tr> | + | <tr><td>AP1</td><td>1 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer1</a> </td></tr> |
| - | <tr><td>AP2</td><td>2 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> primer2</a></td></tr> | + | <tr><td>AP2</td><td>2 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer2</a></td></tr> |
| - | <tr><td>AP3</td><td>3 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> primer3</a></td></tr> | + | <tr><td>AP3</td><td>3 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer3</a></td></tr> |
| - | <tr><td>AP4</td><td>4 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> primer4</a></td></tr> | + | <tr><td>AP4</td><td>4 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer4</a></td></tr> |
<tr><td>AP5</td><td>1 of 4 primers used to create the amiRNA Bet construct</td><td></td></tr> | <tr><td>AP5</td><td>1 of 4 primers used to create the amiRNA Bet construct</td><td></td></tr> | ||
<tr><td>AP6</td><td>2 of 4 primers used to create the amiRNA Bet construct</td><td></td></tr> | <tr><td>AP6</td><td>2 of 4 primers used to create the amiRNA Bet construct</td><td></td></tr> | ||
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<tr><td>AP11</td><td>Forward primer for Bet v1 Antisense</td><td><a href="Bet1.txt">Bet1 Antisense Forward</a></td></tr> | <tr><td>AP11</td><td>Forward primer for Bet v1 Antisense</td><td><a href="Bet1.txt">Bet1 Antisense Forward</a></td></tr> | ||
<tr><td>AP12</td><td>Reverse primer for Bet v1 Antisense</td><td><a href="Bet1.txt"> Bet1 Antisense Reverse</a></td></tr> | <tr><td>AP12</td><td>Reverse primer for Bet v1 Antisense</td><td><a href="Bet1.txt"> Bet1 Antisense Reverse</a></td></tr> | ||
| - | <tr><td>AP13</td><td>Forward primer for LTP Sense</td><td></td></tr> | + | <tr><td>AP13</td><td>Forward primer for LTP Sense</td><td><a href="LTP.txt"> LTP Sense Forward</a></td></tr> |
| - | <tr><td>AP14</td><td>Reverse primer for LTP Sense</td><td></td></tr> | + | <tr><td>AP14</td><td>Reverse primer for LTP Sense</td><td><a href="LTP.txt">LTP Sense Reverse</a></td></tr> |
| - | <tr><td>AP15</td><td>Forward primer for LTP Antisense</td><td></td></tr> | + | <tr><td>AP15</td><td>Forward primer for LTP Antisense</td><td><a href="LTP.txt">LTP Antisense Forward</a></td></tr> |
| - | <tr><td>AP16</td><td>Reverse primer for LTP Antisense</td><td></td></tr> | + | <tr><td>AP16</td><td>Reverse primer for LTP Antisense</td><td><a href="LTP.txt">LTP Antisense Reverse</a></td></tr> |
| - | <tr><td>AP17</td><td>Forward primer for Ger Sense</td><td></td></tr> | + | <tr><td>AP17</td><td>Forward primer for Ger Sense</td><td><a href="Ger.txt">Ger Sense Forward</a></td></tr> |
| - | <tr><td>AP18</td><td>Reverse primer for Ger Sense</td><td></td></tr> | + | <tr><td>AP18</td><td>Reverse primer for Ger Sense</td><td><a href="Ger.txt">Ger Sense Reverse</a></td></tr> |
| - | <tr><td>AP19</td><td>Forward primer for Ger Antisense</td><td></td></tr> | + | <tr><td>AP19</td><td>Forward primer for Ger Antisense</td><td><a href="Ger.txt">Ger Antisense Forward</a></td></tr> |
| - | <tr><td>AP20</td><td>Reverse primer for Ger Antisense</td><td></td></tr> | + | <tr><td>AP20</td><td>Reverse primer for Ger Antisense</td><td><a href="Ger.txt">Ger Antisense Reverse</a></td></tr> |
<tr><td>AP21</td><td>Forward Primer for PDK intron</td><td></td></tr> | <tr><td>AP21</td><td>Forward Primer for PDK intron</td><td></td></tr> | ||
<tr><td>AP22</td><td>Reverse Primer for PDK intron</td><td></td></tr> | <tr><td>AP22</td><td>Reverse Primer for PDK intron</td><td></td></tr> | ||
<tr><td>AP23</td><td>Forward Primer to make amiRNA Biobrick compatible</td><td></td></tr> | <tr><td>AP23</td><td>Forward Primer to make amiRNA Biobrick compatible</td><td></td></tr> | ||
<tr><td>AP24</td><td>Reverse Primer to make amiRNA Biobrick compatible</td><td></td></tr> | <tr><td>AP24</td><td>Reverse Primer to make amiRNA Biobrick compatible</td><td></td></tr> | ||
| - | <tr><td>AP25</td><td>1 of 4 primers used to create the amiRNA LTP construct</td><td></td></tr> | + | <tr><td>AP25</td><td>1 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt">LTP primer 1</a></td></tr> |
| - | <tr><td>AP26</td><td>2 of 4 primers used to create the amiRNA LTP construct</td><td></td></tr> | + | <tr><td>AP26</td><td>2 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt">LTP primer 2</a></td></tr> |
| - | <tr><td>AP27</td><td>3 of 4 primers used to create the amiRNA LTP construct</td><td></td></tr> | + | <tr><td>AP27</td><td>3 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt">LTP primer 3</a></td></tr> |
| - | <tr><td>AP28</td><td>4 of 4 primers used to create the amiRNA LTP construct</td><td></td></tr> | + | <tr><td>AP28</td><td>4 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt">LTP primer 4</a></td></tr> |
Revision as of 07:44, 13 October 2010
parts and primers
Allergy
For more information on the allergens visit our "meet the allergens" page| Name | Original Name | Description | Resistance | Sequence |
| A1 | amiRNA GFP | GFP knockdown construct | chloramphenicol | amirnaGFP |
| A2 | amiRNA (Other allergen x) | knockdown construct for x | chloramphenicol | amiRNA x |
| A3 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | chloramphenicol | ihpRNA Bet1 |
| A4 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | ihpRNA LTP |
| A5 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | ihpRNA GER |
Primers
| Name | Description | Sequence |
| AP1 | 1 of 4 primers used to create the amiRNA GFP construct | GFP primer1 |
| AP2 | 2 of 4 primers used to create the amiRNA GFP construct | GFP primer2 |
| AP3 | 3 of 4 primers used to create the amiRNA GFP construct | GFP primer3 |
| AP4 | 4 of 4 primers used to create the amiRNA GFP construct | GFP primer4 |
| AP5 | 1 of 4 primers used to create the amiRNA Bet construct | |
| AP6 | 2 of 4 primers used to create the amiRNA Bet construct | |
| AP7 | 3 of 4 primers used to create the amiRNA Bet construct | |
| AP8 | 4 of 4 primers used to create the amiRNA Bet construct | |
| AP9 | Forward primer for Bet v1 Sense | Bet1 Sense Forward |
| AP10 | Reverse primer for Bet v1 Sense | Bet1 Sense Reverse |
| AP11 | Forward primer for Bet v1 Antisense | Bet1 Antisense Forward |
| AP12 | Reverse primer for Bet v1 Antisense | Bet1 Antisense Reverse |
| AP13 | Forward primer for LTP Sense | LTP Sense Forward |
| AP14 | Reverse primer for LTP Sense | LTP Sense Reverse |
| AP15 | Forward primer for LTP Antisense | LTP Antisense Forward |
| AP16 | Reverse primer for LTP Antisense | LTP Antisense Reverse |
| AP17 | Forward primer for Ger Sense | Ger Sense Forward |
| AP18 | Reverse primer for Ger Sense | Ger Sense Reverse |
| AP19 | Forward primer for Ger Antisense | Ger Antisense Forward |
| AP20 | Reverse primer for Ger Antisense | Ger Antisense Reverse |
| AP21 | Forward Primer for PDK intron | |
| AP22 | Reverse Primer for PDK intron | |
| AP23 | Forward Primer to make amiRNA Biobrick compatible | |
| AP24 | Reverse Primer to make amiRNA Biobrick compatible | |
| AP25 | 1 of 4 primers used to create the amiRNA LTP construct | LTP primer 1 |
| AP26 | 2 of 4 primers used to create the amiRNA LTP construct | LTP primer 2 |
| AP27 | 3 of 4 primers used to create the amiRNA LTP construct | LTP primer 3 |
| AP28 | 4 of 4 primers used to create the amiRNA LTP construct | LTP primer 4 |
