Team:Yale/Our Project/Notebook/Week 2

(Difference between revisions)

Revision as of 03:23, 20 October 2010

iGEM Yale

Monday 6/14--Primer design, redo of Friday's growth assay
Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived
Wednesday 6/16--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions
Thursday 6/17--more minimal media work and meeting, started BL21 culture
Friday 6/18--plasmid pSB74 arrived (!), already in some unspecified E coli, so plated them out. Transformed promoters (constitutive & IPTG inducible), terminator, and repressor (for inducible promoter) into BL21. Also ran BL21 copper growth assays for wide and narrow concentrations ranges. June the 19th--in which it is revealed that there was a transformation-fail Redid transformation, this time into LE392, set aside pSB74 containing cells in fridge
Sunday 6/20--in evening inoculate 5 mL liquid cultures

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 <li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 7">week 7</a></li>
 <li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 8">week 8</a></li>
+<li><a href="https://2010.igem.org/Team:Yale/Our Project/Protocols">protocols</a></li>
 <li><a href="https://2010.igem.org/Team:Yale/Our Project/Safety">safety</a></li>
+<li><a href="https://2010.igem.org/Team:Yale/Our Project/Future Work">future work</a></li>
 <li><a href="https://2010.igem.org/Team:Yale/Our Project/Applications">applications</a></li>
 </ul>