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Team:Panama/24 August 2010
From 2010.igem.org
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| + | We performed the E. coli transformation, with plasmid (chloramphenicol resistance) + RBS + promoter. | ||
| + | This time we used NEB protocol. | ||
| + | |||
[[Image:24-8.JPG|200px|thumb|left|alt text]] | [[Image:24-8.JPG|200px|thumb|left|alt text]] | ||
| + | |||
| + | We made a miniprep and ran in agarose gel 1%. | ||
| + | |||
| + | 1. Hind III marker (1 uL ladder, 2 uL loading, 7 uL H2O). | ||
| + | 2. Sample (5 uL ligation + 2 uL loading). | ||
Revision as of 19:09, 14 October 2010
We performed the E. coli transformation, with plasmid (chloramphenicol resistance) + RBS + promoter. This time we used NEB protocol.
We made a miniprep and ran in agarose gel 1%.
1. Hind III marker (1 uL ladder, 2 uL loading, 7 uL H2O). 2. Sample (5 uL ligation + 2 uL loading).
