Team:Yale/LabNotebook/Cu

From 2010.igem.org

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(Oct 12, 2010; Standard curve troubleshooting)
(Oct 12, 2010; Standard curve troubleshooting)
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<biblio>
<biblio>
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PMID=11063915
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#Paper1 pmid=11063915
</biblio>
</biblio>

Revision as of 17:19, 12 October 2010

General description of goal of this part of the project.

Contents


Oct 06, 2010; Making stock solutions

Generated the stock solutions for the Followed the standard curve protocol.

  • Made a 100 mM stock solution of BCS.
  • Made two CuSO4 dilution series, in LB and MM, from 10 mM Cu to 10 pM.

Oct 07, 2010; Bacterial growth assays, Cu ppt

# Sample ID Date and Time 1 (nm) 1 (Abs)
1o/n culture10/7/2010 11:16:10 AM6000.854
2MM t010/7/2010 11:44:45 AM6000.002
3LB t010/7/2010 11:31:06 AM6000.089
4LB t110/7/2010 12:47:55 PM6000.189
5MM t110/7/2010 12:45:13 PM6000.002
6MM t210/7/2010 1:41:51 PM6000.004
7LB t210/7/2010 1:43:38 PM6000.450
8MM t310/7/2010 2:44:50 PM6000.001
9LB t310/7/2010 2:46:09 PM6000.761
10MM t410/7/2010 3:53:12 PM6000.005
11LB t410/7/2010 3:54:36 PM6001.064

Oct 12, 2010; Standard curve troubleshooting

<biblio>

  1. Paper1 pmid=11063915

</biblio>

Useful links

LabNotebook

[http://meta.wikimedia.org/wiki/Help:Wikitext_examples Wiki formatting help]

[http://excel2wiki.net/ Excel table to Wiki format tool]