Team:Waterloo

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Superbugs, or antibiotic resistant microorganisms, are microbes that have become resistant to traditional treatments. These types of infections are difficult to diagnose, treat, and eradicate, making the healing process time consuming and resource intensive. The native quorum-sensing unit from S.aureus (the Agr system), will be introduced into a non-pathogenic strain of E.coli. The E.coli will then effectively have the ability to eavesdrop on the activity of the pathogenic organism and emit an indication of the magnitude of the infection in the form of RFP. Using sensitivity tuners the system can be designed such that the response will occur at an exact level, when the size of the population poses a threat to the host. Upon a positive result from a diagnosis, further tests could be done to specify whether MRSA (methicillin-resistant S. aureus) or MSSA (Methicillin-sensitive S. aureus) are present.
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!align="center"|[[Team:Waterloo|Home]]
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!align="center"|[[Team:Waterloo/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Waterloo Official Team Profile]
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!align="center"|[[Team:Waterloo/Project|Project]]
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!align="center"|[[Team:Waterloo/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Waterloo/Modeling|Modeling]]
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!align="center"|[[Team:Waterloo/Human|Human Practices]]
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                <h1>UW iGEM Wiki</h1>
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                  Superbugs, or antibiotic resistant microorganisms, are microbes that have become resistant to traditional treatments. These types of infections are difficult to diagnose, treat, and eradicate, making the healing process time consuming and resource intensive. The native quorum-sensing unit from S.aureus (the Agr system), will be introduced into a non-pathogenic strain of E.coli. The E.coli will then effectively have the ability to eavesdrop on the activity of the pathogenic organism and emit an indication of the magnitude of the infection in the form of RFP. Using sensitivity tuners the system can be designed such that the response will occur at an exact level, when the size of the population poses a threat to the host. Upon a positive result from a diagnosis, further tests could be done to specify whether MRSA (methicillin-resistant S. aureus) or MSSA (Methicillin-sensitive S. aureus) are present.
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          <div class="aContentTop"></div>
 +
          <div id="acmid51" class="aContentMid"></div>
 +
          <div class="aContentBottom"></div>
 +
        </div>
 +
        <div id="acmenu5" class="aContentMidWrapVNav">
 +
          <div class="aContentTop"></div>
 +
          <div class="aContentMid">
 +
              <div class="aContentMenuOption" ></div>
 +
              <div class="aContentMenuOption" onmouseover="aContentMenuHover(1,true)" onmouseout="aContentMenuHover(1,false)" onmousedown="aContentMenuSelect(1)">Notes</div>
 +
              <div class="aContentMenuOption" onmouseover="aContentMenuHover(2,true)" onmouseout="aContentMenuHover(2,false)" onmousedown="aContentMenuSelect(2)">Misc</div>
 +
          </div>
 +
          <div class="aContentBottom"></div>
 +
        </div>
 +
        <div id="acmid5" class="aContentMidWrap">
 +
          <div class="aContentTop"></div>
 +
          <div id="acmid52" class="aContentMid">
 +
            <div class="aContentMidSub"></div>
 +
            <div id="acmid5sub1" class="aContentMidSub"></div>
 +
            <div id="acmid5sub2" class="aContentMidSub"></div>           
 +
          </div>
 +
          <div class="aContentBottom"></div>
 +
        </div>
 +
        <div class="aContentRight">
 +
          <div class="aContentTop"></div>
 +
          <div id="acmid53" class="aContentMid"></div>
 +
          <div class="aContentBottom"></div>
 +
        </div>
 +
      </div>
 +
 
 +
</div>
 +
<div id="uwfooter"></div>
 +
 
 +
<script type="text/javascript">
 +
/**
 +
* Code by Jon Eubank - joneubank@gmail.com - Oct 27, '010
 +
* For use by UWaterloo iGEM team 2010.
 +
*/
 +
 
 +
/* *** Variables *** */
 +
 
 +
//Note: a = short for accordion
 +
var mainContent;
 +
var aContent = [];
 +
var aContentMenu = [];
 +
var aContentMenuWrap = [];
 +
var aContentMenuOptions = [];
 +
var aContentOptions = [];
 +
var aMid = [];
 +
var aHeader = [];
 +
var aHeaderWrap = [];
 +
var aSelected;
 +
var aLength;
 +
var aContentMenuSelected = [];
 +
 
 +
var slideInterval = [];
 +
 
 +
/* *** Invariables! *** */
 +
//by invariable I mean these are as close to constants as we get in js, set these once then leave them be.
 +
var aHeaderWidth = 42;
 +
var aContentMenuWidth = [0, 0, 125, 110, 170, 110];//first 0 is placeholder, then home-project-team-parts-notes
 +
var aContentBorderWidth = 7;
 +
 
 +
//banner heights follow, match them to the CSS stylesheet, important for window resize
 +
var topSpace = 85;
 +
var bottomSpace = 45;
 +
 
 +
//for animation:
 +
var t=15; //interval call rate (in ms)
 +
var slideSpeed=4.5; //divisor for slideTab movement... lower means faster!
 +
 
 +
/* *** Initialization Code *** */
 +
 
 +
function init(accordionLength){
 +
 
 +
  mainContent = document.getElementById("accordion");
 +
  aLength = accordionLength;
 +
 
 +
 
 +
  for(j=1;j<=aLength;j++){
 +
   
 +
    aContent[j] = document.getElementById("ac"+j);
 +
   
 +
    var tempNode = document.getElementById("ah"+j);
 +
    aHeader[j]=tempNode.getElementsByTagName('div');
 +
    aHeaderWrap[j]=tempNode;
 +
    aMid[j] = [];
 +
    aMid[j][0] = document.getElementById("acmid"+j);
 +
   
 +
    for (k=1;k<=3;k++){
 +
      aMid[j][k] = document.getElementById("acmid"+j+k);//poor code, fix if you get a chance - there are only ids of acmid+j+2, 1 and 3 have been removed... really there should be a much better identifier
 +
    }
 +
   
 +
    aContentMenu[j] = [];
 +
    aContentMenu[j] = document.getElementById("acmenu"+j).getElementsByTagName('div');
 +
    aContentMenuWrap[j] = document.getElementById("acmenu"+j);
 +
 
 +
    //fetch content menu options
 +
    aContentMenuOptions[j]=[];
 +
    aContentMenuOptions[j] = aContentMenu[j][1].getElementsByTagName('div');
 +
    aContentOptions[j]=[];
 +
    aContentOptions[j] = aMid[j][2].getElementsByTagName('div');
 +
    aContentOptions[j][1].style.display = "inline";
 +
   
 +
    //Next few lines are to force initialization for content in every tab through aContentMenuSelect
 +
    aSelected = j;//setting aSelected as something makes it pass the checks in aSelect when called after this block
 +
    aContentMenuSelected[aSelected] = 1;
 +
    if (aContentMenuOptions[aSelected].length > 1) {
 +
      aContentMenuOptions[aSelected][aContentMenuSelected[aSelected]].className = "aContentMenuOptionSelected";
 +
    }
 +
  }
 +
 
 +
  aSelect(1);//Open to home tab
 +
 
 +
  //fit to screen
 +
  windowResize();
 +
 
 +
  //set doc to autoresize with the window
 +
  window.onresize=new Function('windowResize()');
 +
}
 +
 
 +
/* *** Event Handling Function(s) *** */
 +
 
 +
function windowResize(){
 +
 
 +
  var mainHeight = window.innerHeight - topSpace - bottomSpace;
 +
 
 +
  //resize heights of mainContent, each mid div, each aContent
 +
  mainContent.style.height = mainHeight + "px";
 +
  for (j = 1; j <= aLength; j++) {
 +
    aHeader[j][1].style.height = (mainHeight - 24) + "px";
 +
    aContent[j].style.height = (mainHeight) + "px";
 +
    aMid[j][2].style.overflow = "auto";
 +
    var newHeight = (mainHeight - aContentBorderWidth*2) + "px";
 +
    aMid[j][1].style.height = newHeight;
 +
    aMid[j][2].style.height = newHeight;
 +
    aMid[j][3].style.height = newHeight;
 +
    aContentMenu[j][1].style.height = newHeight;
 +
  }
 +
 
 +
  //resize widths of open content tab's sub components
 +
  var maxWidth = mainContent.offsetWidth;
 +
  aContent[aSelected].style.width= (maxWidth-aLength*aHeaderWidth)+"px";
 +
  aMid[aSelected][0].style.width = (aContent[aSelected].offsetWidth-aContentBorderWidth*2-aContentMenuWidth[aSelected])+"px";
 +
 
 +
  //this line sets the content of each tab to a set width based on the window size (the content stays hidden because aMidSelected[0] for the non active tabs is 0px;
 +
  for (j = 1; j <= aLength; j++){
 +
    aMid[j][2].style.width = (maxWidth - aContentBorderWidth*2 -aContentMenuWidth[j]-aLength*aHeaderWidth) + "px";
 +
  }
 +
  //set vertical sub nav width to that specified in the invariables
 +
  aContentMenu[aSelected][1].style.width = aContentMenuWidth[aSelected];
 +
  aContentMenuWrap[aSelected].style.width = aContentMenuWidth[aSelected];
 +
}
 +
 
 +
 
 +
/* *** Functions *** */
 +
 
 +
//to be called when a tab is selected:
 +
//sets intervals to open new tab and close old tab, changes class names to cause selected colours
 +
function aSelect(index){
 +
  if(aSelected!=index){
 +
    if(aSelected){
 +
      if (slideInterval[aSelected]) {
 +
        clearInterval(slideInterval[aSelected]);
 +
      }
 +
      var temp = aSelected;
 +
      slideInterval[temp] = setInterval(function(){slideTab(temp,-1)},t);
 +
      aHeader[aSelected][0].parentNode.className = "aHeader";
 +
      aMid[aSelected][2].style.overflow = "hidden";
 +
    }
 +
   
 +
    aSelected = index;
 +
    aHeader[aSelected][0].parentNode.className = "aHeaderSelected";
 +
    scrollHeight = 0;
 +
    if(aContent[aSelected].childNodes[1]){
 +
      aContent[aSelected].childNodes[1].style.margin = scrollHeight + "px 0px 0px 0px";
 +
    }
 +
    if (slideInterval[aSelected]) {
 +
      clearInterval(slideInterval[aSelected]);
 +
    }
 +
    var temp2 = aSelected;
 +
    slideInterval[temp2] = setInterval(function(){slideTab(temp2,1)},t);
 +
  }
 +
}
 +
 
 +
//tab opening and closing, animated.
 +
function slideTab(tab, direction){
 +
  var maxWidth = mainContent.offsetWidth-aLength*aHeaderWidth;
 +
  var change;// =(maxWidth-aContent[tab].offsetWidth);
 +
 
 +
  if(direction<0){
 +
    //change rate is a fraction of the remaining space, so fast at first, slow at end, round up so it is gauranteed to close at the end
 +
    change = Math.ceil((aContent[tab].offsetWidth)/slideSpeed)*direction + aContent[tab].offsetWidth;
 +
    if (change < aContentBorderWidth*2)change=0;
 +
  }
 +
  else {
 +
    //purpose is to set the opening tab to the full size needed for the window, so that we don't see accidental blank space to the right of the accordion menu from resizing issues
 +
    change = maxWidth;
 +
    for(j=1;j<=aLength;j++){
 +
      if (j != tab) {
 +
        change -= aContent[j].offsetWidth;
 +
      }
 +
    }
 +
  }
 +
  //apply change to tab width
 +
  aContent[tab].style.width = change + "px";
 +
 
 +
  //set the width of the content tab's sub div's
 +
  //this if checks if the content tab is more narrow than the conent sub menu,
 +
  if (aContent[tab].offsetWidth < aContentMenuWidth[tab]) {
 +
    var newWidth = (aContent[tab].offsetWidth - aContentBorderWidth * 2) + "px";
 +
  //  aContentMenu[tab][1].style.width = newWidth;
 +
  //  aContentMenu[tab][3].style.width = newWidth;
 +
    aContentMenuWrap[tab].style.width = newWidth;
 +
    aMid[tab][0].style.width = "0px";
 +
  }
 +
  else {
 +
    aContentMenu[tab][1].style.width = aContentMenuWidth[tab] + "px";
 +
    aContentMenuWrap[tab].style.width = aContentMenuWidth[tab] + "px";
 +
    aMid[tab][0].style.width = (aContent[tab].offsetWidth - aContentBorderWidth * 2 - aContentMenuWidth[tab]) + "px";
 +
  }
 +
 
 +
 
 +
 
 +
  //break conditions
 +
if(direction >0){
 +
  if (aContent[tab].offsetWidth >= maxWidth) {
 +
    aContent[tab].style.width = maxWidth+ "px";
 +
  //  aContentMenu[tab][1].style.width = aContentMenuWidth[tab] +"px";
 +
    aContentMenuWrap[tab].style.width = aContentMenuWidth[tab] +"px";
 +
    aMid[tab][2].style.overflow = "auto";
 +
    clearInterval(slideInterval[tab]);
 +
  }}else if (direction < 0){if(aContent[tab].offsetWidth <= 0){
 +
    aContent[tab].style.width = 0 + "px";
 +
    clearInterval(slideInterval[tab]);
 +
    }}
 +
}
 +
 
 +
//onmouseover and onmouseout events, called in HTML, change class names to apply hover colours
 +
function aHover(index, hover){
 +
  if (index != aSelected) {
 +
   
 +
    if (hover) {
 +
      aHeader[index][0].parentNode.className = "aHeaderHover";
 +
    }
 +
    else {
 +
      aHeader[index][0].parentNode.className = "aHeader";
 +
    }
 +
  }
 +
}
 +
 
 +
function aContentMenuSelect(index){
 +
  if (aContentMenuSelected[aSelected]) {
 +
    if (aContentMenuSelected[aSelected] != index) {
 +
      aContentMenuOptions[aSelected][aContentMenuSelected[aSelected]].className = "aContentMenuOption";
 +
      aContentOptions[aSelected][aContentMenuSelected[aSelected]].style.display = "none";
 +
    }
 +
   
 +
  }
 +
  aContentMenuSelected[aSelected] = index;
 +
  aContentMenuOptions[aSelected][aContentMenuSelected[aSelected]].className = "aContentMenuOptionSelected";
 +
  aContentOptions[aSelected][aContentMenuSelected[aSelected]].style.display = "inline";
 +
}
 +
 
 +
function aContentMenuHover(index, hover){
 +
  if (index != aContentMenuSelected[aSelected]){
 +
    if (hover) {
 +
      aContentMenuOptions[aSelected][index].className = "aContentMenuOptionHover";
 +
    }
 +
    else {
 +
      aContentMenuOptions[aSelected][index].className = "aContentMenuOption";
 +
    }
 +
  }
 +
}
 +
    init(5);
 +
</script>
 +
 
 +
</html>

Revision as of 17:16, 24 October 2010

UW iGEM Wiki

Superbugs, or antibiotic resistant microorganisms, are microbes that have become resistant to traditional treatments. These types of infections are difficult to diagnose, treat, and eradicate, making the healing process time consuming and resource intensive. The native quorum-sensing unit from S.aureus (the Agr system), will be introduced into a non-pathogenic strain of E.coli. The E.coli will then effectively have the ability to eavesdrop on the activity of the pathogenic organism and emit an indication of the magnitude of the infection in the form of RFP. Using sensitivity tuners the system can be designed such that the response will occur at an exact level, when the size of the population poses a threat to the host. Upon a positive result from a diagnosis, further tests could be done to specify whether MRSA (methicillin-resistant S. aureus) or MSSA (Methicillin-sensitive S. aureus) are present.
Project
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1
2
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Retrieved from "http://2010.igem.org/Team:Waterloo"