Team:Newcastle/15 June 2010

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Revision as of 09:48, 17 June 2010

Nanodrop protocol

Nanodrop can be used to measure the DNA, RNA and protein

1. Select Nanodrop program from the desktop
2. To clean Nanodrop, add a drop of water on the spectrometer and press blank
3. After cleaning, wipe the water off
4. To equalizen the equipment, add 3μl of the buffer used in the sample and press blank
5. Wipe the buffer off
6. To measure sample, add 3μl of the sample and press measure
7. If dealing with multiple samples, clean the equipment with water at regular intervals
8. After measurement, clean the equipment with a drop of water on the spectrometer and press blank

Gel electrophoresis

Gel electrophoresis can used to separate DNA, RNA, or protein molecules by molecular weight using an electric field applied to a gel matrix

1. Make up 1% agarose gel (1g of agarose in 100ml of TAE buffer) and transfer 60ml of molten agarose gel into the gel tray with 3μl of Safeview
2. Wait for 30min to allow the gel to harden
3. Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerge
4. Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder was used for analysing DNA
5. Loading buffer was then added together with the sample before loading onto the gel matrix
6. Run gel at 90V until separation is achieved and visualize using the gelDoc