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Team:BCCS-Bristol/Wetlab
From 2010.igem.org
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| + | '''09/07/2010 - Day One''' | ||
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| + | Made two different competent strains of ''E.coli'', and attempted transformations on both using BBa_I13522 (pTet GFP) from the kit - prepared 6 plates: 1 positive control for each strain, 1 negative control for each strain and 1 test transformation plate for each strain. The positive control was carried out using a plasmid of known quantity. Ampicillin was used for selection. | ||
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| + | '''12/07/2010 - Day Two''' | ||
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| + | No growth on test plates. | ||
Revision as of 10:14, 19 July 2010
Wet lab
09/07/2010 - Day One
Made two different competent strains of E.coli, and attempted transformations on both using BBa_I13522 (pTet GFP) from the kit - prepared 6 plates: 1 positive control for each strain, 1 negative control for each strain and 1 test transformation plate for each strain. The positive control was carried out using a plasmid of known quantity. Ampicillin was used for selection.
12/07/2010 - Day Two
No growth on test plates.
