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Team:TU Delft/Project/rbs-characterization/parts
From 2010.igem.org
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Our constructs used for measurements: | Our constructs used for measurements: | ||
<partinfo>K398500 SequenceAndFeatures</partinfo> | <partinfo>K398500 SequenceAndFeatures</partinfo> | ||
| - | <partinfo>K398501 | + | <partinfo>K398501 SequenceAndFeatures</partinfo> |
| - | <partinfo>K398502 | + | <partinfo>K398502 SequenceAndFeatures</partinfo> |
| - | <partinfo>K398503 | + | <partinfo>K398503 SequenceAndFeatures</partinfo> |
| - | <partinfo>K398504 | + | <partinfo>K398504 SequenceAndFeatures</partinfo> |
Revision as of 14:13, 8 October 2010
BioBricks, the making of
BBa_I13401 was PCR amplified using the universal primers G00100 and G00101. The purified PCR product was assembled into one of the Anderson RBS plasmids provided in the distribution plates by means of 2-way ligations.
Transformation into competent Top10 E.coli cells yielded positives, as determined by fluorescence analysis.
The cells were cultured over 18 hours in 96-well plates using a Gen5 fluorescence and absorbance plate reader, LB with ampicillin was the culture medium used in these experiments. Only the results obtained during exponential growth phase were taken into account.
Parts
Our constructs used for measurements: <partinfo>K398500 SequenceAndFeatures</partinfo> <partinfo>K398501 SequenceAndFeatures</partinfo> <partinfo>K398502 SequenceAndFeatures</partinfo> <partinfo>K398503 SequenceAndFeatures</partinfo> <partinfo>K398504 SequenceAndFeatures</partinfo>
