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Team:HokkaidoU Japan/Test
From 2010.igem.org
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== Dr. E.coli : The smallest protein injector in the world == | == Dr. E.coli : The smallest protein injector in the world == | ||
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There are several transfection methods to introduce nucleic acid into cells, however methods to introduce proteins directly into cells are complicated. | There are several transfection methods to introduce nucleic acid into cells, however methods to introduce proteins directly into cells are complicated. | ||
Revision as of 02:09, 8 October 2010
since 13 Jul 2010
since 1 Aug 2010
Dr. E.coli : The smallest protein injector in the world
There are several transfection methods to introduce nucleic acid into cells, however methods to introduce proteins directly into cells are complicated.
Unlike introducing DNA, which changes cell genetic makeup permenantly, proteins can act at pinpoint acuracy. And afterwards be degraded leaving no permanent information in the target cell,
Our mission was to find an easy way to introduce desired proteins in the cell. Speficaly into eucaryotic cell. In order to do this we chose Type 3 Secreation System. Find in Salmonella and EPEc. These bacteria are pathogenic and working with them is problematic. To make things easier and safer we ordered DNA fragment which contains T3SS`s syringe part. And introduce it into E.coli.
We also needed protein to secreat. We desined it by ataching secreation signal to desired protein. Our choice was GFP. Also added NLS to make it go to nucleus.
