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Team:Groningen/6 September 2010
From 2010.igem.org
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'''Assembly of biobricks in pSB1C3-backbone for submission to parts registry''' ''by Maarten'' | '''Assembly of biobricks in pSB1C3-backbone for submission to parts registry''' ''by Maarten'' | ||
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Control restrictions with EcoRI and PstI on constructs: | Control restrictions with EcoRI and PstI on constructs: | ||
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| - | The gel that was run did not show any DNA present on the gel. | + | The gel that was run did not show any DNA present on the gel. Experiment was continued on [[Team:Groningen/8 September 2010 | 8 September]]. |
Revision as of 21:13, 7 October 2010
Assembly of biobricks in pSB1C3-backbone for submission to parts registry by Maarten
Control restrictions with EcoRI and PstI on constructs:
| 2 µL construct |
| 2 µL buffer O |
| 0,5 µL EcoRI |
| 0,5 µL PstI |
| 15 µL MQ |
| Construct | Expected band sizes (bp) |
|---|---|
| pSB1C3-C | 2031, 848 |
| pSB1C3-DC | 2031, 872 |
| pSB1C3-E | 2031, 317 |
| pSB1C3-H | 2031, 302 |
| pSB1C3-Sortase | 2031, 689 |
| pSB1C3-RFP | 2031, 1069 |
The gel that was run did not show any DNA present on the gel. Experiment was continued on 8 September.
