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Team:Panama/Project
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| - | There is considerable interest among bio-industries in bioremediation products such as Rhamnolipids. Rhamnolipids as biosurfactants are important in the remediation of oil spill areas. The cleanup of the Exxon Valdez oil spill | + | There is considerable interest among bio-industries in bioremediation products such as Rhamnolipids. Rhamnolipids as biosurfactants are important in the remediation of oil spill areas. The cleanup of the Exxon Valdez oil spill with rhamnolipids as biosurfactants was too expensive and complicated, therefore impractical for large-scale bioremediation. However, advances genetic engineering and synthetic biology offer a viable solution to oil spill pollution clean up. In this work we use genetic engineering as a tool to integrate genetic parts by the biobrick assembly standard protocol of iGEM to develop a biobrick-friendly for rhamnosiltransferase 1 complex (rhlAB) gene expression in ''Escherichia coli'' for standardized rhamnolipid production. Our biobrick integrates a promoter, a RBS (ribosomal binding site), our gene sequence of rh1AB isolated from ''Pseudomonas aeruginosa'', a GFP reporter and a terminator. All the parts fit into a plasmid backbone that can be transformed into ''E. coli'' strains, which can then produce rhamnolipids. |
== Project Details== | == Project Details== | ||
Revision as of 18:24, 21 October 2010
Republic of Panama
Situated on the isthmus connecting North and South America, it
is bordered by Costa Rica to the northwest, Colombia to the southeast,
the Caribbean Sea to the north and the Pacific Ocean to the south.
Republic of Panama
Situated on the isthmus connecting North and South America, it is bordered by Costa Rica to the northwest, Colombia to the southeast, the Caribbean Sea to the north and the Pacific Ocean to the south.
iGEM PANAMA
In this picture: Carolina, Yisett, Claudio, Nicole, Lorena,
Grimaldo, Natasha, Laura, Ernesto, Dra. Carmenza, Dr. Rao, Ezequiel
iGEM PANAMA
In this picture: Carolina, Yisett, Claudio, Nicole, Lorena, Grimaldo, Natasha, Laura, Ernesto, Dra. Carmenza, Dr. Rao, Ezequiel
iGEM PANAMA
In this picture: Yisett, Silke, Yaraví, Carlos, Dr. Patrick
Nee.
iGEM PANAMA
In this picture: Yisett, Silke, Yaraví, Carlos, Dr. Patrick Nee.
iGEM PANAMA
In this picture: Leyda, Zeuz, Laura
iGEM PANAMA
In this picture: Leyda, Zeuz, Laura
iGEM PANAMA
Labs.
iGEM PANAMA
Labs.
Contents |
Overall project
Standardization of the Rhamnosiltransferase 1 gene complex (rhlAB) into a Biobrick-friendly for rhamnolipid production in E. coli.
There is considerable interest among bio-industries in bioremediation products such as Rhamnolipids. Rhamnolipids as biosurfactants are important in the remediation of oil spill areas. The cleanup of the Exxon Valdez oil spill with rhamnolipids as biosurfactants was too expensive and complicated, therefore impractical for large-scale bioremediation. However, advances genetic engineering and synthetic biology offer a viable solution to oil spill pollution clean up. In this work we use genetic engineering as a tool to integrate genetic parts by the biobrick assembly standard protocol of iGEM to develop a biobrick-friendly for rhamnosiltransferase 1 complex (rhlAB) gene expression in Escherichia coli for standardized rhamnolipid production. Our biobrick integrates a promoter, a RBS (ribosomal binding site), our gene sequence of rh1AB isolated from Pseudomonas aeruginosa, a GFP reporter and a terminator. All the parts fit into a plasmid backbone that can be transformed into E. coli strains, which can then produce rhamnolipids.
