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Team:WashU/Notebook/Yeast
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| + | A practice transformation was preformed at the Cohen Lab with Ilaria. The OD600 was 0.651 which was low, but used in order to speed up the experiment. The PMKV005 loxKanlox Plasmid (McCusker 2005 Yeast, 21: 163-171) was used into transform wildtype yeast. The plates used were Kan positive plates created on 6/24. However it was later found that these plates might not correctly select for drug resistance. Two plates were made, one with the transformant and one with a control. The attached procedure was used and annotated during the experiment | ||
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Revision as of 18:32, 4 October 2010
Week of 6/21
6/24
plated Yeast Gal-YFP MATa, trp1, leu2::GAL1-YFP URA3/Kanr and Yeast YF4 MATa Prototroph on a Kan plate and a YPD plate
Started a liquid culture of Yeast YF4 MATa Prototroph in YPD
Week of 6/28
6/29
A practice transformation was preformed at the Cohen Lab with Ilaria. The OD600 was 0.651 which was low, but used in order to speed up the experiment. The PMKV005 loxKanlox Plasmid (McCusker 2005 Yeast, 21: 163-171) was used into transform wildtype yeast. The plates used were Kan positive plates created on 6/24. However it was later found that these plates might not correctly select for drug resistance. Two plates were made, one with the transformant and one with a control. The attached procedure was used and annotated during the experiment
