Team:HokkaidoU Japan/Notebook/October4

From 2010.igem.org

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{|
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Sequencing of 2 samples, RBS + Arabinose
 +
 
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{|border="5"
|-
|-
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| 12:00
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| 9:00
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| aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa<br>aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
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|
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*Reserve the sequencer for use at 16:00 if posible
 +
|-
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| 12:10
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|
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*Consult colonie PCR to choose constructs for Sequencing.
 +
*Miniprep
 +
*MasterMix
 +
*PCR for sequencing, use 100 up and 200 down primers
 +
|-
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| 12:55~14:55
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|
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*PCR
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|-
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| 15:00~15:40
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|  
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*Ethanol precipitation
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*Melting in Hi-DI
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|-
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| 16:00
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|
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*Start sequencing
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|-
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| 17:00
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|
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*Retrieve sequence
|}
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Revision as of 17:07, 4 October 2010

Notebook

Sequencing of 2 samples, RBS + Arabinose

9:00
  • Reserve the sequencer for use at 16:00 if posible
12:10
  • Consult colonie PCR to choose constructs for Sequencing.
  • Miniprep
  • MasterMix
  • PCR for sequencing, use 100 up and 200 down primers
12:55~14:55
  • PCR
15:00~15:40
  • Ethanol precipitation
  • Melting in Hi-DI
16:00
  • Start sequencing
17:00
  • Retrieve sequence
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Notebook/October4"