Team:UNIPV-Pavia/Calendar/October/settimana1

(Difference between revisions)

Revision as of 11:01, 3 October 2010

OCTOBER: WEEK 1

Glycerol stock for:

Pick of a single non-red colony of INTEIN_C3 and inoculum into 5 ml LB+Cm34. Sample was let grow ON at +37°C, 220rpm.

Miniprep and quantification of:

I55-1bis was digested E-S for about three hours and than gel run/cut.

Colony PCR for I74 (six colonies were picked)

Gel run for PCR samples and I55 (E-S) and cut for the last one.

I74 screening and I55 E-S cut.

Gel extraction and quantification:

Ligation of:

Inoculum of I35 into 5 ml LB+Amp: last quantification after gel extraction was too poor. We eill digest it again E-P.

@@ Line 59: / Line 59: @@
 *I55 (E-S): ng/ul
+Ligation of:
+*I75: I55 (E-S) + I37 (E-X)
+*I76: I55 (E-S) + I53 (E-X)
+*I77: I55 (E-S) + I57 (E-X)
 ----
 Inoculum of I35 into 5 ml LB+Amp: last quantification after gel extraction was too poor. We eill digest it again E-P.