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Team:UNIPV-Pavia/Calendar/October/settimana1
From 2010.igem.org
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==October, 3rd== | ==October, 3rd== | ||
| + | Glycerol stock for: | ||
| + | *I55-1 | ||
| + | *IC-1/2/3/4-4C5 | ||
| + | |||
| + | ---- | ||
| + | Pick of a single non-red colony of INTEIN_C3 and inoculum into 5 ml LB+Cm34. Sample was let grow ON at +37°C, 220rpm. | ||
| + | |||
| + | ---- | ||
| + | Miniprep and quantification of: | ||
| + | *I55-1: 57,4 ng/ul | ||
| + | *I55-1bis: 79,6 ng/ul | ||
| + | |||
| + | I55-1bis was digested E-S for about three hours and than gel run/cut. | ||
| + | |||
| + | ---- | ||
| + | |||
| + | Colony PCR for I74 (six colonies were picked) | ||
| + | |||
| + | Gel run for PCR samples and I55 (E-S) and cut for the last one. | ||
| + | [[Image:UNIPV10_03_10_2010_I74_screen_I55_cut.jpg|thumb|200px|center|I74 screening and I55 E-S cut.]] | ||
| + | |||
| + | ---- | ||
| + | Gel extraction and quantification: | ||
| + | *I55 (E-S): ng/ul | ||
| + | |||
| + | |||
| + | |||
| + | ---- | ||
| + | Inoculum of I35 into 5 ml LB+Amp: last quantification after gel extraction was too poor. We eill digest it again E-P. | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
Revision as of 10:52, 3 October 2010
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