Team:Stockholm/27 September 2010

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===Sequencing===
===Sequencing===
Samples prepared 25/9 were sent for sequencing. Sequencing information added to the 25/9 notebook page.
Samples prepared 25/9 were sent for sequencing. Sequencing information added to the 25/9 notebook page.
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== Mimmi ==
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=== Over expression ===
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{|
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| Start cultures
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|-
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| *3ml LB<sub>AMP</sub> + tip from glycerol stock
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|-
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| *Grow ON in 37&deg;C, 225rpm
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|-
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| '''DNA'''
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|-
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| pEX.SOD
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|-
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| pEX.yCCS
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|-
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| pEX.SOD.his
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|-
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| pEX.his.SOD
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|}

Revision as of 22:51, 25 October 2010

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Contents

Andreas

Cloning and assembly

Transformation results

From 25/9 transformations

Good colony yield on all plates. Good white:red colony ratio on pEX plates (Constructs 1, 2, 3 and 4).

Colony PCR

Picked colonies for colony PCR.

  1. pEX.N-LMWP⋅SOD⋅His (K): 1-2
  2. pEX.N-TAT⋅SOD⋅His: 1-2
  3. pEX.N-Tra10⋅SOD⋅His: 1-2
  4. pEX.N-LMWP⋅SOD⋅His (C): 1-2
  5. pSB1K3.N-LMWP⋅SOD⋅His.RBS.yCCS: 1-4
  6. pSB1K3.N-TAT⋅SOD⋅His.RBS.yCCS: 1-4
  7. pSB1K3.N-Tra10⋅SOD⋅His.RBS.yCCS: 1-4
  8. pSB1C3.N-LMWP⋅SOD⋅His.RBS.yCCS: 1-4
  9. BL21 pEX.N-TAT⋅SOD⋅His 3: 1-2
  10. BL21 pEX.N-TAT⋅SOD⋅His 4: 1-2

Standard colony PCR protocol.

  • Elongation time: 2:00

Gel verification

Gel 1
1 % agarose, 110 V

Gel 2
0.8 % agarose, 90 V

Expected bands

  1. 744 bp
  2. 735 bp
  3. 765 bp
  4. 744 bp
  5. 1645 bp
  6. 1636 bp
  7. 1666 bp
  8. 1645 bp
  9. 735 bp
  10. 735 bp

Results
Gels run too far (no data). New gels will be run tomorrow.

Sequencing

Samples prepared 25/9 were sent for sequencing. Sequencing information added to the 25/9 notebook page.



Mimmi

Over expression

Start cultures
*3ml LBAMP + tip from glycerol stock
*Grow ON in 37°C, 225rpm
DNA
pEX.SOD
pEX.yCCS
pEX.SOD.his
pEX.his.SOD
Retrieved from "http://2010.igem.org/Team:Stockholm/27_September_2010"