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Team:Cambridge/Gibson/Protocol
From 2010.igem.org
(Difference between revisions)
(→Master mix) |
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| Line 10: | Line 10: | ||
|Volume/µl | |Volume/µl | ||
|- | |- | ||
| - | |Taq ligase 40u/µl | + | |Taq ligase (40u/µl) |
|50 | |50 | ||
|- | |- | ||
| Line 16: | Line 16: | ||
|100 | |100 | ||
|- | |- | ||
| - | |T5 exonuclease 1u/µl | + | |T5 exonuclease (1u/µl) |
|2 | |2 | ||
|- | |- | ||
| - | |Phusion polymerase 2u/µl | + | |Phusion polymerase (2u/µl) |
|6.25 | |6.25 | ||
|- | |- | ||
| - | |Nuclease | + | |Nuclease-free water |
|216.75 | |216.75 | ||
|- | |- | ||
Revision as of 09:56, 30 September 2010
Gibson Assembly: Protocols
Master mix
| Volume/µl | |
| Taq ligase (40u/µl) | 50 |
| 5x isothermal buffer | 100 |
| T5 exonuclease (1u/µl) | 2 |
| Phusion polymerase (2u/µl) | 6.25 |
| Nuclease-free water | 216.75 |
| =375 |
