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Revision as of 20:36, 27 September 2010 (view source) Jaretz (Talk | contribs) (→Sequenced BioBricks) ← Older edit		Revision as of 11:48, 30 September 2010 (view source) Jaretz (Talk | contribs) Newer edit →
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	* <partinfo>K389010</partinfo> (not fully completed)		* <partinfo>K389010</partinfo> (not fully completed)
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		+	* <partinfo>K389011</partinfo> (not fully completed)
	* <partinfo>K389012</partinfo> (not fully completed)		* <partinfo>K389012</partinfo> (not fully completed)
	* <partinfo>K389013</partinfo> (not fully completed)		* <partinfo>K389013</partinfo> (not fully completed)
		+
		+	* <partinfo>K389015</partinfo> (not fully completed)
		+
		+	* <partinfo>K389016</partinfo> (not fully completed)
	===Other BioBricks===		===Other BioBricks===

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Revision as of 11:48, 30 September 2010

Contents 1 Tested BioBricks 1.1 <partinfo>K238008</partinfo>: virA 1.2 <partinfo>BBa_K238011</partinfo>: vir-promotor 1.3 <partinfo>P1010</partinfo>: ccdB-gene 2 Sequenced BioBricks 2.1 Own BioBricks 2.2 Other BioBricks

Tested BioBricks

We tested the following BioBricks:

<partinfo>K238008</partinfo>: virA

We made a restriction analysis and sequenced parts of this BioBrick. There were problems - more information within a short time.

<partinfo>BBa_K238011</partinfo>: vir-promotor

We made a restriction analysis and sequenced parts of this BioBrick.

<partinfo>P1010</partinfo>: ccdB-gene

We transformed this BioBrick into Escherichia coli JM109, DH5α, TOP10 and DB3.1. E. coli JM109 and DH5α seem to be ccdB resistant because there were as much colonies after P1010 transformation as observed with DB3.1. The P1010 works as expected in E. coli TOP10 (no colonies after transformation) and DB3.1 (lawn after transformation).

Sequenced BioBricks

Own BioBricks

The sequencing of the following of our own BioBricks was succesful and lead to the expected results:

• <partinfo>K389001</partinfo> (not fully completed)

• <partinfo>K389002</partinfo>

• <partinfo>K389003</partinfo>

• <partinfo>K389004</partinfo>

• <partinfo>K389005</partinfo>

• <partinfo>K389010</partinfo> (not fully completed)

• <partinfo>K389011</partinfo> (not fully completed)

• <partinfo>K389012</partinfo> (not fully completed)

• <partinfo>K389013</partinfo> (not fully completed)

• <partinfo>K389015</partinfo> (not fully completed)

• <partinfo>K389016</partinfo> (not fully completed)

Other BioBricks

• <partinfo>K238008</partinfo> sequencing gave negative results - infos in registry are not correct!

• <partinfo>K238011</partinfo> sequencing gave negative results - infos in registry are not correct!