Team:Utah State

From 2010.igem.org

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    <title>Utah State University - iGEM 2010</title>
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''The aim of the Utah State University iGEM project is to develop flexible and adaptable systems for manufacturing cellular products using the standardized BioBrick system in a wide range of species. First, we altered the standard BioBrick vector pSB1A3 to allow for the insertion of genomic integration sequences from Synechocystis sp. PCC 6803 and enable the insertion of BioBrick constructs directly into the host genome. This integration vector is easily adapted for use in other species through the exchange of the species-specific integration sequences and the ability to alter the selection marker, if necessary. This vector will facilitate exploitation of advantageous characteristics of a wide range of organisms, including photosynthetic carbon assimilation. The BioBrick toolbox was expanded to allow the utilization of Synechocystis as a host species, with the identification and characterization of ribosome binding sites, constitutive promoters, and promoters responsive to light, dark, nitrogen stress, heat stress, and circadian rhythms. Secretion systems for Synechocystis were adapted to the BioBrick standard, and the cross-species function of E. coli secretion tags was investigated. Project success will facilitate the expansion of BioBrick-coded products into multiple organisms and the characterization of further useful genetic elements in those species.''
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[[Image:charles.jpg|center|frame|Dr. Charles Miller|50px]]
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[[Image:brad.jpg|center|frame|Brad Henrie|50px]]
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!align="center"|[[Team:Utah_State|Home]]
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!align="center"|[[Team:Utah_State/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Utah_State Official Team Profile]
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!align="center"|[[Team:Utah_State/Project|Project]]
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!align="center"|[[Team:Utah_State/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Utah_State/Modeling|Modeling]]
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!align="center"|[[Team:Utah_State/Notebook|Notebook]]
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!align="center"|[[Team:Utah_State/Safety|Safety]]
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    <td></td>
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    <td><a name="top"><img width="950" src="https://static.igem.org/mediawiki/2010/5/5e/Utah_State_logo.png" /></a></td>
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    <td align="center" ><a class="mainLinks" href="" >Project Summary</a> </td>
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    <td align="center" ><a class="mainLinks" href="" >Meet the Team</a> </td>
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    <td align="center"><a class="mainLinks" href=""  >Sponsors</a> </td>
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    <td align="center"><a class="mainLinks" href=""  >Something else</a> </td>
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          <td bgcolor="#006633"><p align="center" style="font-size:20px; color:#FFFFFF;"> <em>CyanoBricks</em></p></td>
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          <td><a class="sideLinks" href="" title="choosing our project">Project</a></td>
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          <td><a class="sideLinks" href="" title="Design">Design</a></td>
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          <td><a class="sideLinks" href="" title="Construction">Construction</a></td>
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          <td><a class="sideLinks" href="" title="Software Modeling">Modeling</a></td>
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          <td><a class="sideLinks" href="" title="Testing">Testing</a></td>
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          <td><a class="sideLinks" href="" title="Protocols">Protocols</a></td>
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          <td><a class="sideLinks" href="" title="Results">Results</a></td>
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    <td valign="top"><span style="font-size:23px">Welcome to the Utah State University's 2010 Wiki</span>
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      <hr />
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      <align="justified">
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      <p>The future of synthetic biology lies in expanding our ability to engineer genes in new organisms.
 +
        Our project develops a system to engineer the genome of the photosynthetic cyanobacterium Synechocystis sp. PCC6803, 
 +
        establishes expression standards for this species, and adds a set of characterized Synechocystis promoters and ribosome
 +
        binding sites to the BioBrick toolbox. We developed a BioBrick vector that can be used to assemble parts and devices in E.
 +
        coli. Upon transformation into Synechocystis, it integrates the device directly into the genome through homologous
 +
        recombination. We utilized genes that were activated under a variety of conditions, from those responding to heat stress to
 +
        ones oscillating under a circadian rhythm. The promoters and ribosome binding sites were converted into BioBrick-compatible
 +
        parts, and subsequently characterized. Our success will enable the use of existing parts in new species, and will expand the
 +
        range of devices that can be built. </p>
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          <td style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size:18px">CyanoBricks</span>
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            <hr />
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            <div style="float:left; margin:5px;"> <img src="https://static.igem.org/mediawiki/2010/b/bc/Hands_on.png" alt="Picture Here!" /> </div>
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            <p> <em>CyanoBricks</em> Description. </p></td>
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          <td style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size:18px">Another thing!</span>
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            <hr />
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            <div style="float:left; margin:5px;"> <img src="https://static.igem.org/mediawiki/2010/f/f8/Usu_people.png" alt="Another picture here!" /> </div>
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            <p>Something else here.</p></td>
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          <td style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size:18px">Some Links</span>
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            <hr />
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            <br/>
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            <p><a href="" title="Link 1">Link 1</a></p>
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            <p><a href="" title="Link 2">Link 2</a></p>
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            <p><a href="" title="Link 3">Link 3</a> </p>
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          <td style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size:18px">Room for somthing else</span>
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            <hr />
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                  Anything can go here!
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Revision as of 03:36, 27 September 2010

Utah State University - iGEM 2010

Project Summary Meet the Team Sponsors Something else

CyanoBricks
Project
Design
Construction
Modeling
Testing
Protocols
Results
Welcome to the Utah State University's 2010 Wiki

The future of synthetic biology lies in expanding our ability to engineer genes in new organisms. Our project develops a system to engineer the genome of the photosynthetic cyanobacterium Synechocystis sp. PCC6803, establishes expression standards for this species, and adds a set of characterized Synechocystis promoters and ribosome binding sites to the BioBrick toolbox. We developed a BioBrick vector that can be used to assemble parts and devices in E. coli. Upon transformation into Synechocystis, it integrates the device directly into the genome through homologous recombination. We utilized genes that were activated under a variety of conditions, from those responding to heat stress to ones oscillating under a circadian rhythm. The promoters and ribosome binding sites were converted into BioBrick-compatible parts, and subsequently characterized. Our success will enable the use of existing parts in new species, and will expand the range of devices that can be built.

CyanoBricks
Picture Here!

CyanoBricks Description.

Another thing!
Another picture here!

Something else here.
Some Links

Link 1

Link 2

Link 3

Room for somthing else
Anything can go here!

Retrieved from "http://2010.igem.org/Team:Utah_State"