Team:RMIT Australia/Notebook
From 2010.igem.org
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<div id=" .2F21_May_2010"></div> | <div id=" .2F21_May_2010"></div> | ||
- | <div id=" .2F28_June_2010"> | + | <div id=" .2F28_June_2010"> |
+ | ===28 June=== | ||
Did OH&S training and PC2 training | Did OH&S training and PC2 training | ||
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</div> | </div> | ||
- | <div id=" .2F29_June_2010"> | + | <div id=" .2F29_June_2010"> |
+ | ===29 June=== | ||
Began research on what we were going to use to promote taq | Began research on what we were going to use to promote taq | ||
</div> | </div> | ||
- | <div id=" .2F30_June_2010"> | + | <div id=" .2F30_June_2010"> |
+ | ===30 June=== | ||
Looked at T7 with a Lac operon as a promotore of taq | Looked at T7 with a Lac operon as a promotore of taq | ||
</div> | </div> | ||
- | <div id=" .2F13_July_2010"> | + | <div id=" .2F13_July_2010"> |
+ | ===13 July=== | ||
Designed Primers for Taq into backbone | Designed Primers for Taq into backbone | ||
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</div> | </div> | ||
- | <div id=" .2F14_July_2010"> | + | <div id=" .2F14_July_2010"> |
+ | ===14 July=== | ||
Looked at prices of Hotels in Boston | Looked at prices of Hotels in Boston | ||
</div> | </div> | ||
- | <div id=" .2F19_July_2010"> | + | <div id=" .2F19_July_2010"> |
+ | ===19 July=== | ||
began looking at the structure of taq, its thermostability | began looking at the structure of taq, its thermostability | ||
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</div> | </div> | ||
- | <div id=" .2F21_July_2010"> | + | <div id=" .2F21_July_2010"> |
+ | ===21 July=== | ||
sent in sequence of wild type taq to iTasser | sent in sequence of wild type taq to iTasser | ||
</div> | </div> | ||
- | <div id=" .2F23_July_2010"> | + | <div id=" .2F23_July_2010"> |
+ | ===23 July=== | ||
Checked thermostability of the mutations that will be done to Taq using Expasy | Checked thermostability of the mutations that will be done to Taq using Expasy | ||
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Sent in sequence of taq with mutations at 485, 515 and 540 to iTasser | Sent in sequence of taq with mutations at 485, 515 and 540 to iTasser | ||
- | <div id=" .2F3_August_2010"> | + | <div id=" .2F3_August_2010"> |
+ | ===3 August=== | ||
Had iGEM team meeting | Had iGEM team meeting | ||
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</div> | </div> | ||
- | <div id=" .2F5_August_2010"> | + | <div id=" .2F5_August_2010"> |
+ | ===5 August=== | ||
Meet up with Damian, discussed different strategies to use in modelling, began using YASARA to do our Modelling | Meet up with Damian, discussed different strategies to use in modelling, began using YASARA to do our Modelling | ||
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</div> | </div> | ||
- | <div id=" .2F10_August_2010"> | + | <div id=" .2F10_August_2010"> |
+ | ===10 August=== | ||
Had iGEM team meeting | Had iGEM team meeting | ||
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</div> | </div> | ||
- | <div id=" .2F11_August_2010"> | + | <div id=" .2F11_August_2010">===11 August=== |
PCR of Taq | PCR of Taq | ||
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</div> | </div> | ||
- | <div id=" .2F16_August_2010"> | + | <div id=" .2F16_August_2010"> |
+ | ===16 August=== | ||
Collaborated with the Sheffield team by participating in an over the phone survery about Human Practices | Collaborated with the Sheffield team by participating in an over the phone survery about Human Practices | ||
- | |||
</div> | </div> | ||
+ | <div id=" .2F17_August_2010"> | ||
+ | ===17 August=== | ||
+ | |||
had team meeting | had team meeting | ||
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</div> | </div> | ||
- | <div id=" .2F18_August_2010"> | + | <div id=" .2F18_August_2010"> |
+ | ===18 August=== | ||
called few companies seeking sponsorship | called few companies seeking sponsorship | ||
enquired for booking flights and accomadation | enquired for booking flights and accomadation | ||
- | </div id=" .2F19_August_2010"> | + | </div id=" .2F19_August_2010"> |
+ | ===19 August=== | ||
Working on comparing TAQ wild structure to the TAQ Mutated structure to see that there are no major differences, which will mean that TAQ is inactive but will retain it's thermostability at high temperatures. | Working on comparing TAQ wild structure to the TAQ Mutated structure to see that there are no major differences, which will mean that TAQ is inactive but will retain it's thermostability at high temperatures. | ||
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|} | |} | ||
- | <div id=" .2F20_August_2010"> | + | <div id=" .2F20_August_2010"> |
+ | ===20 August=== | ||
+ | |||
Did two PCR reaction and a transformation | Did two PCR reaction and a transformation | ||
The first PCR we ran was a touch down PCR. Temperatures ranged from 69 to 64. | The first PCR we ran was a touch down PCR. Temperatures ranged from 69 to 64. | ||
The second PCR was a two step PCR. | The second PCR was a two step PCR. | ||
- | <div id=" .2F30_August_2010"> | + | <div id=" .2F30_August_2010"> |
+ | ===30 August=== | ||
Ordered the TAQ polymerase from Mr. Gene. Should have it in 15 days. | Ordered the TAQ polymerase from Mr. Gene. Should have it in 15 days. | ||
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After the meeting we all are sitting together for a writing session. | After the meeting we all are sitting together for a writing session. | ||
- | <div id=" .2F31_August_2010"> | + | <div id=" .2F31_August_2010"> |
+ | ===31 August=== | ||
Spent the morning organising our flights | Spent the morning organising our flights | ||
In the afternoon we all sat together for a writing session | In the afternoon we all sat together for a writing session | ||
- | <div id=" .2F1_September_2010"> | + | <div id=" .2F1_September_2010"> |
+ | ===1 September=== | ||
We all sat together for another writing session. | We all sat together for another writing session. | ||
- | <div id=" .2F7_September_2010"> | + | <div id=" .2F7_September_2010"> |
+ | ===7 September=== | ||
Had a team meeting | Had a team meeting | ||
- | <div id=" .2F14_September_2010"> | + | <div id=" .2F14_September_2010"> |
+ | ===14 September=== | ||
Had a team meeting and made a lab time table, we also set out tasks which need to be done by the next meeting | Had a team meeting and made a lab time table, we also set out tasks which need to be done by the next meeting | ||
- | + | *Update the wiki | |
- | + | * Finalise the form for the ethics committee for pennstate | |
- | + | * Call up possible sponsors | |
- | + | * Collect Tshirts | |
- | + | * Work on abstract and team Roster | |
- | + | * research heat liable bonds | |
- | + | * order peptides | |
- | + | * chat with emilio about possible bio assays | |
- | + | * work on making the promotor part | |
- | <div id=" .2F15_September_2010"> | + | <div id=" .2F15_September_2010"> |
+ | ===15 September=== | ||
'''Mutagenesis Quikchange XL kit''' | '''Mutagenesis Quikchange XL kit''' | ||
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Dpn1 digest (2 µl) for 2hrs | Dpn1 digest (2 µl) for 2hrs | ||
- | <div id=" .2F16_September_2010"> | + | <div id=" .2F16_September_2010"> |
+ | ===16 September=== | ||
2 µl of the mutagenesis product into 100 µl of competent cells | 2 µl of the mutagenesis product into 100 µl of competent cells | ||
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- | <div id=" .2F17_September_2010"> | + | <div id=" .2F17_September_2010"> |
+ | ===17 September=== | ||
- finalized project abstract | - finalized project abstract | ||
- | <div id=" .2F19_September_2010"> | + | <div id=" .2F19_September_2010"> |
+ | ===19 September=== | ||
- sent off project abstract to HQ | - sent off project abstract to HQ |
Revision as of 01:11, 24 September 2010
Notebook
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20 AugustDid two PCR reaction and a transformation The first PCR we ran was a touch down PCR. Temperatures ranged from 69 to 64. The second PCR was a two step PCR. 30 AugustOrdered the TAQ polymerase from Mr. Gene. Should have it in 15 days. Meeting today at 10am to discuss project budget and costings. After the meeting we all are sitting together for a writing session. 31 AugustSpent the morning organising our flights In the afternoon we all sat together for a writing session 1 SeptemberWe all sat together for another writing session. 7 SeptemberHad a team meeting 14 SeptemberHad a team meeting and made a lab time table, we also set out tasks which need to be done by the next meeting
15 SeptemberMutagenesis Quikchange XL kit
We optimised for low 260/280
First mutagenesis was to turn the -35 site in the promoter region into an Afi II restriction site. Dpn1 digest (2 µl) for 2hrs 16 September2 µl of the mutagenesis product into 100 µl of competent cells Plate 50 µl, 100 µl and 150 µl samples.
17 September- finalized project abstract 19 September- sent off project abstract to HQ |