Team:HokkaidoU Japan/Materials And Methods

From 2010.igem.org

(Difference between revisions)
(→Materials And Methods)
(→Materials And Methods)
Line 122: Line 122:
<!-- Row 14 -->
<!-- Row 14 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074]
-
<!--      Description -->|
+
<!--      Description -->|T7 promoter (strong promoter from T7 bacteriophage)
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|1-6N
<!--              Well -->|1-6N
-
<!--            Length -->|
+
<!--            Length -->|46 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1AK8
|-
|-
<!-- Row 15 -->
<!-- Row 15 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 BBa_F2621]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 BBa_F2621]
-
<!--      Description -->|
+
<!--      Description -->|3OC6HSL Receiver Device
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|2-21H
<!--              Well -->|2-21H
-
<!--            Length -->|
+
<!--            Length -->|1158 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1A2
|-
|-
<!-- Row 16 -->
<!-- Row 16 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J45200 BBa_J45200]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J45200 BBa_J45200]
-
<!--      Description -->|
+
<!--      Description -->|Banana odor generator
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|2-5F
<!--              Well -->|2-5F
-
<!--            Length -->|
+
<!--            Length -->|1801 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1AT3
|-
|-
<!-- Row 17 -->
<!-- Row 17 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K112000 BBa_K112000]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K112000 BBa_K112000]
-
<!--      Description -->|
+
<!--      Description -->|T4 holin, complete CDS, berkeley standard
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|3-15B
<!--              Well -->|3-15B
-
<!--            Length -->|
+
<!--            Length -->|657 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|BBa_K112950
|-
|-
<!-- Row 18 -->
<!-- Row 18 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K112022 BBa_K112022]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K112022 BBa_K112022]
-
<!--      Description -->|
+
<!--      Description -->|Lambda phage lysis device - no promoter
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|3-24E
<!--              Well -->|3-24E
-
<!--            Length -->|
+
<!--            Length -->|1499 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|BBa_K112954
|-
|-
<!-- Row 19 -->
<!-- Row 19 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_I0500 BBa_I0500]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_I0500 BBa_I0500]
-
<!--      Description -->|
+
<!--      Description -->|Inducible pBad/araC promoter
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|3-20B
<!--              Well -->|3-20B
-
<!--            Length -->|
+
<!--            Length -->|1210 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB2K3
|-
|-
<!-- Row 20 -->
<!-- Row 20 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084014 BBa_K084014]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084014 BBa_K084014]
-
<!--      Description -->|
+
<!--      Description -->|3OC6HSL Sender Device
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|2-2B
<!--              Well -->|2-2B
-
<!--            Length -->|
+
<!--            Length -->|869 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1A2
|-
|-
<!-- Row 21 -->
<!-- Row 21 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_P1002 BBa_P1002]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_P1002 BBa_P1002]
-
<!--      Description -->|
+
<!--      Description -->|ampicillin resistance cassette
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|2-21B
<!--              Well -->|2-21B
-
<!--            Length -->|
+
<!--            Length -->|943 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1A1
|-
|-
<!-- Row 22 -->
<!-- Row 22 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_I765001 BBa_I765001]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_I765001 BBa_I765001]
-
<!--      Description -->|
+
<!--      Description -->|UV promoter
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|1-21B
<!--              Well -->|1-21B
-
<!--            Length -->|
+
<!--            Length -->|76 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1A2
|-
|-
<!-- Row 23 -->
<!-- Row 23 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J06702 BBa_J06702]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J06702 BBa_J06702]
-
<!--      Description -->|
+
<!--      Description -->|mCherry, bacterial with RBS and forward terminator
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|2-8E
<!--              Well -->|2-8E
-
<!--            Length -->|
+
<!--            Length -->|869 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1A2
|-
|-
<!-- Row 24 -->
<!-- Row 24 -->

Revision as of 03:14, 23 September 2010

Materials And Methods

BioBrick No. Discription Distribution Well

BioBricks Used
BioBrick No. Description Distribution Well No. Length Plasmid
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] double terminator (B0010-B0012) Spring 2010 Distribution 1-23L 129bp pSB1AK3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040] wild-type GFP Spring 2010 Distribution 1-14K 720 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K098993 BBa_K098993] heat sensitive cI QPI with low promoter Spring 2010 Distribution 3-1G 935 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I6316 BBa_I6316] QPI Curve Test I0500.E0420.I0500.Q04511.E0430 Spring 2010 Distribution 2-1M 5243 pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J45119 BBa_J45119] Wintergreen odor enzyme (BMST1) generator Spring 2010 Distribution 2-5B 1230 bp pSB1AT3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840 BBa_E0840] GFP generator Spring 2010 Distribution 1-12O 878 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13001 BBa_I13001] Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG Spring 2010 Distribution 1-24G 881 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522] pTet GFP Spring 2010 Distribution 2-8A 937 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010 BBa_E1010] **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) Spring 2010 Distribution 1-18F 681 bp pSB2K3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450] RFP Coding Device Spring 2010 Distribution 1-5A pSB1K3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450] RFP Coding Device Spring 2010 Distribution 1-1A pSB1A10
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K098995 BBa_K098995] heat sensitive cI QPI with high promoter Spring 2010 Distribution 3-1E 935 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450] RFP Coding Device Spring 2010 Distribution 1-3A pSB1C3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074] T7 promoter (strong promoter from T7 bacteriophage) Spring 2010 Distribution 1-6N 46 bp pSB1AK8
[http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 BBa_F2621] 3OC6HSL Receiver Device Spring 2010 Distribution 2-21H 1158 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J45200 BBa_J45200] Banana odor generator Spring 2010 Distribution 2-5F 1801 bp pSB1AT3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K112000 BBa_K112000] T4 holin, complete CDS, berkeley standard Spring 2010 Distribution 3-15B 657 bp BBa_K112950
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K112022 BBa_K112022] Lambda phage lysis device - no promoter Spring 2010 Distribution 3-24E 1499 bp BBa_K112954
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I0500 BBa_I0500] Inducible pBad/araC promoter Spring 2010 Distribution 3-20B 1210 bp pSB2K3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084014 BBa_K084014] 3OC6HSL Sender Device Spring 2010 Distribution 2-2B 869 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_P1002 BBa_P1002] ampicillin resistance cassette Spring 2010 Distribution 2-21B 943 bp pSB1A1
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I765001 BBa_I765001] UV promoter Spring 2010 Distribution 1-21B 76 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J06702 BBa_J06702] mCherry, bacterial with RBS and forward terminator Spring 2010 Distribution 2-8E 869 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:]
[http://partsregistry.org/wiki/index.php?title=Part:]
[http://partsregistry.org/wiki/index.php?title=Part:]
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Materials_And_Methods"