Team:HokkaidoU Japan/Materials And Methods

From 2010.igem.org

(Difference between revisions)
(→Materials And Methods)
(→Materials And Methods)
Line 66: Line 66:
<!-- Row 7 -->
<!-- Row 7 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13001 BBa_I13001]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13001 BBa_I13001]
-
<!--      Description -->|
+
<!--      Description -->|Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|1-24G
<!--              Well -->|1-24G
-
<!--            Length -->|
+
<!--            Length -->|881 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1A2
|-
|-
<!-- Row 8 -->
<!-- Row 8 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522]
-
<!--      Description -->|
+
<!--      Description -->|pTet GFP
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|2-8A
<!--              Well -->|2-8A
-
<!--            Length -->|
+
<!--            Length -->|937 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1A2
|-
|-
<!-- Row 9 -->
<!-- Row 9 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010 BBa_E1010]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010 BBa_E1010]
-
<!--      Description -->|
+
<!--      Description -->|**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|1-18F
<!--              Well -->|1-18F
-
<!--            Length -->|
+
<!--            Length -->|681 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB2K3
|-
|-
<!-- Row 10 -->
<!-- Row 10 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450]
-
<!--      Description -->|
+
<!--      Description -->|RFP Coding Device
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|1-5A
<!--              Well -->|1-5A
<!--            Length -->|
<!--            Length -->|
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1K3
|-
|-
<!-- Row 11 -->
<!-- Row 11 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450]
-
<!--      Description -->|
+
<!--      Description -->|RFP Coding Device
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|1-1A
<!--              Well -->|1-1A
<!--            Length -->|
<!--            Length -->|
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1A10
|-
|-
<!-- Row 12 -->
<!-- Row 12 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K098995 BBa_K098995]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K098995 BBa_K098995]
-
<!--      Description -->|
+
<!--      Description -->|heat sensitive cI QPI with high promoter
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|3-1E
<!--              Well -->|3-1E
-
<!--            Length -->|
+
<!--            Length -->|935 bp
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1A2
|-
|-
<!-- Row 13 -->
<!-- Row 13 -->
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450]
<!--      BioBrick No. -->|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450]
-
<!--      Description -->|
+
<!--      Description -->|RFP Coding Device
<!--      Distribution -->|Spring 2010 Distribution
<!--      Distribution -->|Spring 2010 Distribution
<!--              Well -->|1-3A
<!--              Well -->|1-3A
<!--            Length -->|
<!--            Length -->|
-
<!--          Plasmid -->|
+
<!--          Plasmid -->|pSB1C3
|-
|-
<!-- Row 14 -->
<!-- Row 14 -->

Revision as of 02:51, 23 September 2010

Materials And Methods

BioBrick No. Discription Distribution Well

BioBricks Used
BioBrick No. Description Distribution Well No. Length Plasmid
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] double terminator (B0010-B0012) Spring 2010 Distribution 1-23L 129bp pSB1AK3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040] wild-type GFP Spring 2010 Distribution 1-14K 720 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K098993 BBa_K098993] heat sensitive cI QPI with low promoter Spring 2010 Distribution 3-1G 935 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I6316 BBa_I6316] QPI Curve Test I0500.E0420.I0500.Q04511.E0430 Spring 2010 Distribution 2-1M 5243 pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J45119 BBa_J45119] Wintergreen odor enzyme (BMST1) generator Spring 2010 Distribution 2-5B 1230 bp pSB1AT3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840 BBa_E0840] GFP generator Spring 2010 Distribution 1-12O 878 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13001 BBa_I13001] Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG Spring 2010 Distribution 1-24G 881 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522] pTet GFP Spring 2010 Distribution 2-8A 937 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010 BBa_E1010] **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) Spring 2010 Distribution 1-18F 681 bp pSB2K3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450] RFP Coding Device Spring 2010 Distribution 1-5A pSB1K3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450] RFP Coding Device Spring 2010 Distribution 1-1A pSB1A10
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K098995 BBa_K098995] heat sensitive cI QPI with high promoter Spring 2010 Distribution 3-1E 935 bp pSB1A2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450] RFP Coding Device Spring 2010 Distribution 1-3A pSB1C3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074] Spring 2010 Distribution 1-6N
[http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 BBa_F2621] Spring 2010 Distribution 2-21H
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J45200 BBa_J45200] Spring 2010 Distribution 2-5F
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K112000 BBa_K112000] Spring 2010 Distribution 3-15B
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K112022 BBa_K112022] Spring 2010 Distribution 3-24E
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I0500 BBa_I0500] Spring 2010 Distribution 3-20B
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084014 BBa_K084014] Spring 2010 Distribution 2-2B
[http://partsregistry.org/wiki/index.php?title=Part:BBa_P1002 BBa_P1002] Spring 2010 Distribution 2-21B
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I765001 BBa_I765001] Spring 2010 Distribution 1-21B
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J06702 BBa_J06702] Spring 2010 Distribution 2-8E
[http://partsregistry.org/wiki/index.php?title=Part:]
[http://partsregistry.org/wiki/index.php?title=Part:]
[http://partsregistry.org/wiki/index.php?title=Part:]
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Materials_And_Methods"