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	= Evaluation for Wet Laboratory =		= Evaluation for Wet Laboratory =
-	== Tasks for our models==	+	== Evaluation goals ==
		+	To determine the best possible parts for the biological implementation of E. lemming, combined molecular models (Light switch - Chemotaxis) have been used to answer specific questions:
-	[[Image:A1.png|center|600px|Sketch for a possible implementation of the controller.]]	+	# Which receptor (Che) protein should be attacked?
-		+	# To which light-sensitive protein (PhyB, PIF3) should the receptor protein be linked?
-	1. '''Estimate CheYp for no tumbling''': we already know from the literature that the tumbling frequency of the bacterium is a function of the concentration of CheY phosphorylated. Therefore, our first task was to determine the concentration of CheYp (defined as the ‘Threshold’) necessary to get a bias (= time not tumbling/total time) of 1 or close enough to 1 so that we could consider that the bacterium exhibits no tumbling (for a given input).<br>	+	# What is a good aspartate concentration?
-	2. '''Construct the light input''': Given the value of CheYp from 1), the second task would be to construct this light input which induces the effect of ‘no tumbling’ (or ‘running straight’). This is to be done by fusing PIF3 (for example) with any of the Che proteins (CheB, CheR, CheZ). The best light input signal is the one for which the bias is close enough to 1 for the highest amount of time (in other words, CheYp stays the most beyond the Threshold). <br>	+	# In what quantity should the anchor and binding light-sensitive protein be chosen?
-	3. Repeat the previous tasks for '''different values of the input concentrations''' (different input values trigger different effects on the bias of the system). <br>	+

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Revision as of 07:46, 27 September 2010

Evaluation for Wet Laboratory

Evaluation goals

To determine the best possible parts for the biological implementation of E. lemming, combined molecular models (Light switch - Chemotaxis) have been used to answer specific questions:

1. Which receptor (Che) protein should be attacked?
2. To which light-sensitive protein (PhyB, PIF3) should the receptor protein be linked?
3. What is a good aspartate concentration?
4. In what quantity should the anchor and binding light-sensitive protein be chosen?