Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/08
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===Electrophoresis=== | ===Electrophoresis=== |
Revision as of 10:15, 22 September 2010
2010/09/08
PCR
<Member>
Hitomi
<Sample>
- 5
- 1+2(ligation 2010/08/31)
- 4+5(ligation 2010/08/31)
- 7+8(ligation 2010/09/06)
<Protocol>
See Protocol2 (https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)
- Tube(temperature in annealing)
- 1.(71.0,70.5,70.0℃)
- 2.(72.0,71.5℃)
- 3.(70.0℃)
- 4.(70.0℃)
- Total 12 tubes.
Electrophoresis
<Member>
mizuki, Hitomi
<Sample>
PCR products.
- 5H
- 5M
- 5L
- 1-2H
- 1-2L
- 4-5
- 7-8
<Protocol>
See Protocol8
(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)
<Result>
File:Matsuura 2010-09-08 18hr 09min.tif