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Revision as of 10:11, 22 September 2010 (view source) Mariko (Talk | contribs) (→PCR) ← Older edit		Revision as of 10:13, 22 September 2010 (view source) Mariko (Talk | contribs) (→Electrophoresis) Newer edit →
Line 33:		Line 33:
	<Member>		<Member>
		+
	mizuki, Hitomi		mizuki, Hitomi
Line 38:		Line 39:
	<Sample>		<Sample>
		+	PCR products.
		+	:*5H
		+	:*5M
		+	:*5L
		+	:*1-2H
		+	:*1-2L
		+	:*4-5
		+	:*7-8
	<Protocol>		<Protocol>
		+
		+	See Protocol8
		+
		+	(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)

---

Revision as of 10:13, 22 September 2010

2010/09/08

PCR

<Member>

Hitomi

<Sample>

• 5
• 1+2(ligation 2010/08/31)
• 4+5(ligation 2010/08/31)
• 7+8(ligation 2010/09/06)

<Protocol>

See Protocol2 (https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)

• Tube(temperature in annealing)

1.(71.0,70.5,70.0℃)

2.(72.0,71.5℃)

3.(70.0℃)

4.(70.0℃)

Total 12 tubes.

Electrophoresis

<Member>

mizuki, Hitomi

<Sample>

PCR products.

• 5H
• 5M
• 5L
• 1-2H
• 1-2L
• 4-5
• 7-8

<Protocol>

See Protocol8

(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)

<Result>

File:Matsuura 2010-09-08 18hr 09min.tif