"
Team:HokkaidoU Japan/Protocols
From 2010.igem.org
(Difference between revisions)
(→Protocols) |
|||
| Line 1: | Line 1: | ||
{{Template:HokkaidoU_Japan}} | {{Template:HokkaidoU_Japan}} | ||
=Protocols= | =Protocols= | ||
| + | ==Contents== | ||
* Preparation of Competent cells (''E. coli'' DH5a) | * Preparation of Competent cells (''E. coli'' DH5a) | ||
* Bacterial Transformations | * Bacterial Transformations | ||
| Line 11: | Line 12: | ||
==Preparation of Competent cells (''E. coli'' DH5a)== | ==Preparation of Competent cells (''E. coli'' DH5a)== | ||
| + | * Day 1 | ||
| + | ## Single colony isolation on LB plate | ||
| + | ## incubate the plate for 15-19 hrs at 37C | ||
| + | ## lift a colony into 2 mL LB | ||
| + | |||
| + | |||
| + | |||
==Bacterial Transformations== | ==Bacterial Transformations== | ||
==Mini-prep (Alkaline SDS Method)== | ==Mini-prep (Alkaline SDS Method)== | ||
Revision as of 15:24, 21 September 2010
since 13 Jul 2010
since 1 Aug 2010
Protocols
Contents
- Preparation of Competent cells (E. coli DH5a)
- Bacterial Transformations
- Mini-prep (Alkaline SDS Method)
- PCR
- Restriction Enzyme Digestions
- DNA ligation
- Agarose gel electrophoresis
- Electroporation
Preparation of Competent cells (E. coli DH5a)
- Day 1
- Single colony isolation on LB plate
- incubate the plate for 15-19 hrs at 37C
- lift a colony into 2 mL LB
