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Team:HokkaidoU Japan/Protocols
From 2010.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
* Agarose gel electrophoresis | * Agarose gel electrophoresis | ||
* Electroporation | * Electroporation | ||
| + | |||
| + | ==Preparation of Competent cells (''E. coli'' DH5a)== | ||
| + | ==Bacterial Transformations== | ||
| + | ==Mini-prep (Alkaline SDS Method)== | ||
| + | ==PCR= | ||
| + | ==Restriction Enzyme Digestions== | ||
| + | ==DNA ligation== | ||
| + | ==Agarose gel electrophoresis== | ||
| + | ==Electroporation== | ||
Revision as of 15:01, 21 September 2010
since 13 Jul 2010
since 1 Aug 2010
Protocols
- Preparation of Competent cells (E. coli DH5a)
- Bacterial Transformations
- Mini-prep (Alkaline SDS Method)
- PCR
- Restriction Enzyme Digestions
- DNA ligation
- Agarose gel electrophoresis
- Electroporation
