Team:HokkaidoU Japan/Notebook/August31

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{{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/August30|August 30]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September1|September 1]]</div></div>
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=pUC119のゲル抽&Ligation&Transformation=
=pUC119のゲル抽&Ligation&Transformation=
* pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え,3レーンに流した
* pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え,3レーンに流した

Revision as of 15:01, 18 September 2010

pUC119のゲル抽&Ligation&Transformation

  • pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え,3レーンに流した
  • ゲル抽出(220 mg)
  • 50 uL(500 ng/uL)になったとする

Ligation

なし I M
DNA 2 uL 2 uL 2 uL
ligation solution I 2 uL
Mighty Mix 2 uL
T4 ligase 0.4 uL 0.4 uL
Total 2 uL 4.4 uL 4.4 uL
→Transformation