Team:HokkaidoU Japan/Notebook/August31
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- | {{Template:HokkaidoU_Japan}} | + | {{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/August30|August 30]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September1|September 1]]</div></div> |
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=pUC119のゲル抽&Ligation&Transformation= | =pUC119のゲル抽&Ligation&Transformation= | ||
* pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え,3レーンに流した | * pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え,3レーンに流した |
Revision as of 15:01, 18 September 2010
pUC119のゲル抽&Ligation&Transformation
- pUC119 solution 50 uLに 10 uL 6x Sample Bufferを加え,3レーンに流した
- ゲル抽出(220 mg)
- 50 uL(500 ng/uL)になったとする
Ligation
なし | I | M | |
DNA | 2 uL | 2 uL | 2 uL |
ligation solution I | - | 2 uL | - |
Mighty Mix | - | - | 2 uL |
T4 ligase | - | 0.4 uL | 0.4 uL |
Total | 2 uL | 4.4 uL | 4.4 uL |