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Team:ETHZ Basel/Biology/Molecular Mechanism
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| + | Sourjik and Berg: Localization of components of the chemotaxis machinery of Escheria coli using fluorescent protein fusions. Molecular Biology. 2000; 37:4.] | ||
Revision as of 16:19, 15 September 2010
Contents |
Relevant properties of the chemotaxis pathway
Localization Che proteins
In [1] CheA, CheY and CheZ proteins were fused to YFP in E. coli mutants to determine their localization inside the cell. All three proteins tend to co-localize with methyl accepting chemotaxis proteins (MCPs, the receptor proteins of the chemotaxis pathway) at the membrane, but with different strengths.
- CheA and CheZ nearly only localize at the MCPs.
- CheY has a higher concentration around the MCPs, but is present in significant concentrations in the cytoplasm.
Consistent with common knowledge, CheA was not localized inside the cell in mutants lacking MCPs. CheZ showed no localization in ΔcheA mutants. CheY did not show any significant localization in ΔcheA mutants nor in mutants lacking a significant amount of receptor proteins.
Relevant properties of the PhyB/PIF3 module
Literature
Sourjik and Berg: Localization of components of the chemotaxis machinery of Escheria coli using fluorescent protein fusions. Molecular Biology. 2000; 37:4.]
