Team:HokkaidoU Japan/Notebook/September15

From 2010.igem.org

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(New page: *Observed results of yesterdays transformation **Transformation using heat shock went well **Electroporation transformation failed produce colonies *Did Colony PCR of yesterdays transforme...)
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== Result of yesterdays transformation ==
== Result of yesterdays transformation ==
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Transformation using heat shock went well and produced about 100 colonies. We chose 10 and did colony PCR to check if insert was correct. The insert was Arabinose promoter[[http://partsregistry.org/Part:BBa_I0500 (BBa_I0500)]] and GFP reporter[[http://partsregistry.org/Part:BBa_E0840 (BBa_E0840)]]
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Transformation using heat shock went well and produced about 100 colonies. We chose 10 and did colony PCR to check if insert was correct. The insert was Arabinose promoter[[http://partsregistry.org/Part:BBa_I0500 (BBa_I0500)]] and GFP reporter[http://partsregistry.org/Part:BBa_E0840 (BBa_E0840)]

Revision as of 11:57, 15 September 2010

  • Observed results of yesterdays transformation
    • Transformation using heat shock went well
    • Electroporation transformation failed produce colonies
  • Did Colony PCR of yesterdays transformed colonies
  • Introduced colonies to L(+)Arabinose medium to check if it would show desired function
    • Check for results tomorrow


Result of yesterdays transformation

Transformation using heat shock went well and produced about 100 colonies. We chose 10 and did colony PCR to check if insert was correct. The insert was Arabinose promoterhttp://partsregistry.org/Part:BBa_I0500 (BBa_I0500) and GFP reporter[http://partsregistry.org/Part:BBa_E0840 (BBa_E0840)]

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