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BIOTEC Dresden/Notepad/30 August 2010
From 2010.igem.org
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The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out. Ligation reactions were incubated at room temperature for 1 hour. | The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out. Ligation reactions were incubated at room temperature for 1 hour. | ||
The whole ligation reaction was used for electroporation. | The whole ligation reaction was used for electroporation. | ||
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{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Revision as of 13:50, 24 October 2010
Fusion Protein
The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out. Ligation reactions were incubated at room temperature for 1 hour. The whole ligation reaction was used for electroporation.
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