Team:KAIST-Korea/Project/Methods/PCR Result

From 2010.igem.org

(Difference between revisions)
(→9/6 ~ 9/9 PCR Result)
(→9/6 ~ 9/9 PCR Result)
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<td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">53*C</td><td width = "223px">-</td><td width = "70px">-</td>
<td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">53*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">c</td><td width = "255px">Fusion Antibody receptor</td><td width = "200px">53*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">d</td><td width = "255px">Signal peptide</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">e</td><td width = "255px">Ig-like</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">f</td><td width = "255px">Signal Peptide + Ig-like</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">g</td><td width = "255px">Antibody</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">h</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">53*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">i</td><td width = "255px">B_FGFR1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">j</td><td width = "255px">VB_FGFR1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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Revision as of 15:30, 12 September 2010

 

PCR Result

9/6 ~ 9/9 PCR Result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)2ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



exp 1

SegmentTemperatureResult
aSTAT153*C--
bV_STAT153*C--
cFusion Antibody receptor53*C--
dSignal peptide56*C--
eIg-like56*C--
fSignal Peptide + Ig-like56*C--
gAntibody56*C--
hV_Fusion Antibody Receptor53*C--
iB_FGFR156*C--
jVB_FGFR156*C--
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