Team:KAIST-Korea/Project/Methods/PCR Result

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(Difference between revisions)
(→9/6 ~ 9/9 PCR Result)
(→9/6 ~ 9/9 PCR Result)
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<td>a</td><td>STAT1</td><td>-</td><td>-</td>
<td>a</td><td>STAT1</td><td>-</td><td>-</td>
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<td>V_STAT_F</td><td>50pmole</td>
 
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<td>V_STAT_R</td><td>50pmole</td>
 
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<td>dNTP (dNTP mixture 2.5mM TaKaRa)</td><td>5ul</td>
 
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<td>Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)</td><td>0.25ul</td>
 
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<td>10x buffer (10x cloned Pfu Reaction buffer)</td><td>5ul</td>
 
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<td>DW (3rd sterile water)</td><td>rest</td>
 
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<td>Total</td><td>50ul</td>
 
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Revision as of 15:11, 12 September 2010

 

PCR Result

9/6 ~ 9/9 PCR Result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)2ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



exp 1

SegmentResult
aSTAT1--
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