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Team:USTC/Notebook/Meetings/11 September 2010
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Knightmare (Talk | contribs) (New page: Knightmare： Plasmid extraction: ABJKNTUV on PSB1C3 in old standard with concentration and digestion determination.) |
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Plasmid extraction: ABJKNTUV on PSB1C3 in old standard with concentration and digestion determination. | Plasmid extraction: ABJKNTUV on PSB1C3 in old standard with concentration and digestion determination. | ||
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| + | Jocelyn: | ||
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| + | Double Digest ABJKNTUV on PSB1C3 and Promoter. (0.5h & 1h)=>Ligation =>transformation into TOP10 | ||
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| + | Construct pduAB under new standard.(condition tests:3h+PCR recycle+1h)=>ligation=>transformation into TOP10(on 9.12) | ||
Revision as of 13:32, 11 September 2010
Knightmare：
Plasmid extraction: ABJKNTUV on PSB1C3 in old standard with concentration and digestion determination.
Jocelyn:
Double Digest ABJKNTUV on PSB1C3 and Promoter. (0.5h & 1h)=>Ligation =>transformation into TOP10
Construct pduAB under new standard.(condition tests:3h+PCR recycle+1h)=>ligation=>transformation into TOP10(on 9.12)
