Team:HokkaidoU Japan/NotebookTest

From 2010.igem.org

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<div style="clear:both"></div>
<div style="clear:both"></div>
=Abstract=
=Abstract=
-
===[[Team:HokkaidoU_Japan/Notebook/July12|Monday, July 12, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/July12|Monday, July 12]]===
* Our first experiment, transformation of BioBrick devices.
* Our first experiment, transformation of BioBrick devices.
----
----
-
===[[Team:HokkaidoU_Japan/Notebook/July21|Wednesday, July 21, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/July21|Wednesday, July 21]]===
* Preparation of competent cells
* Preparation of competent cells
-
* Single colony isolation of ''E. coli'' which had been transformed on Monday, July 12, 2010
+
* Single colony isolation of ''E. coli'' which had been transformed on Monday, July 12
-
===[[Team:HokkaidoU_Japan/Notebook/July26|Monday, July 26, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/July26|Monday, July 26]]===
* Cultivation of transformed ''E. coli'' for the next day's miniprep
* Cultivation of transformed ''E. coli'' for the next day's miniprep
-
===[[Team:HokkaidoU_Japan/Notebook/July27|Tuesday, July 27, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/July27|Tuesday, July 27]]===
* Miniprep
* Miniprep
----
----
-
===[[Team:HokkaidoU_Japan/Notebook/August2|Monday, August 2, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August2|Monday, August 2]]===
* Restriction enzyme digestion of plasmids which had been purified by miniprep
* Restriction enzyme digestion of plasmids which had been purified by miniprep
* Electrophoresis assay
* Electrophoresis assay
-
===Tuesday, August 3, 2010===
+
===Tuesday, August 3===
-
===Wednesday, August 4, 2010===
+
===Wednesday, August 4===
-
===Thursday, August 5, 2010===
+
===Thursday, August 5===
-
===Friday, August 6, 2010===
+
===Friday, August 6===
----
----
-
===[[Team:HokkaidoU_Japan/Notebook/August9|Monday, August 9, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August9|Monday, August 9]]===
* Initial amplification of the BioBrick parts (BBa_I14032 "2-11P", BBa_F2621 "2-21H", BBa_K098995 "3-1E" and BBa_E1010 "1-18F") that are used in the construction of concentration-sensitive ''E. coli and heat-sensitive ''E. coli
* Initial amplification of the BioBrick parts (BBa_I14032 "2-11P", BBa_F2621 "2-21H", BBa_K098995 "3-1E" and BBa_E1010 "1-18F") that are used in the construction of concentration-sensitive ''E. coli and heat-sensitive ''E. coli
* These are preliminary experiments before starting the main project
* These are preliminary experiments before starting the main project
-
===[[Team:HokkaidoU_Japan/Notebook/August10|Tuesday, August 10, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August10|Tuesday, August 10]]===
* PCR amplification of the other parts (BBa_F1610 "2-24G", BBa_B0034 "1-2M", BBa_B0015 "1-23L" and BBa_K098995 "3-1E") and electrophoresis to confirm
* PCR amplification of the other parts (BBa_F1610 "2-24G", BBa_B0034 "1-2M", BBa_B0015 "1-23L" and BBa_K098995 "3-1E") and electrophoresis to confirm
-
===[[Team:HokkaidoU_Japan/Notebook/August11|Wednesday, August 11, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August11|Wednesday, August 11]]===
* Preparation for the next day's miniprep
* Preparation for the next day's miniprep
* Estimation of enzyme activity
* Estimation of enzyme activity
* Preparation of the glycerol stocks
* Preparation of the glycerol stocks
-
===[[Team:HokkaidoU_Japan/Notebook/August12|Thursday, August 12, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August12|Thursday, August 12]]===
* Miniprep (1-18F, 2-21H and 2-11P)
* Miniprep (1-18F, 2-21H and 2-11P)
* Electrophoresis of the DNA which had been amplified via PCR and miniprep
* Electrophoresis of the DNA which had been amplified via PCR and miniprep
-
===[[Team:HokkaidoU_Japan/Notebook/August13|Friday, August 13, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August13|Friday, August 13]]===
* PCR amplification of the DNA that we couldn't obtain via miniprep previous day(1-18F)
* PCR amplification of the DNA that we couldn't obtain via miniprep previous day(1-18F)
----
----
-
===[[Team:HokkaidoU_Japan/Notebook/August16|Monday, August 16, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August16|Monday, August 16]]===
* Measurement of restriction enzyme activity
* Measurement of restriction enzyme activity
* Restriction enzyme digestion of the parts(3-1E, 1-2M, 1-18F and 1-23L) and the vector(pSB1C3)
* Restriction enzyme digestion of the parts(3-1E, 1-2M, 1-18F and 1-23L) and the vector(pSB1C3)
-
===[[Team:HokkaidoU_Japan/Notebook/August17|Tuesday, August 17, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August17|Tuesday, August 17]]===
* Purification of the DNA solutions via gel extraction
* Purification of the DNA solutions via gel extraction
* Preparation of agar medium containing 35 ug/mL chloramphenicol
* Preparation of agar medium containing 35 ug/mL chloramphenicol
-
===[[Team:HokkaidoU_Japan/Notebook/August18|Wednesday, August 18, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August18|Wednesday, August 18]]===
* Digestion and gel extraction of 1-2M (retry)
* Digestion and gel extraction of 1-2M (retry)
* 3 piece ligation of 1-18F, 1-23L and pSB1C3
* 3 piece ligation of 1-18F, 1-23L and pSB1C3
* Transformation
* Transformation
-
===[[Team:HokkaidoU_Japan/Notebook/August19|Thursday, August 19, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August19|Thursday, August 19]]===
* 3 piece ligation of 3-1E (heat sensor), 1-2M (ribosome binding site) and pSB1C3 (vector)
* 3 piece ligation of 3-1E (heat sensor), 1-2M (ribosome binding site) and pSB1C3 (vector)
* Ligation that uses only vector pSB1C3 to estimate its efficiency
* Ligation that uses only vector pSB1C3 to estimate its efficiency
-
===[[Team:HokkaidoU_Japan/Notebook/August20|Friday, August 20, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August20|Friday, August 20]]===
* PCR amplification of pSB1A3, pSB1C3 and pSB1T3
* PCR amplification of pSB1A3, pSB1C3 and pSB1T3
----
----
-
===[[Team:HokkaidoU_Japan/Notebook/August23|Monday, August 23, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August23|Monday, August 23]]===
* PCR amplification of pSB1A3, pSB1C3 and pSB1T3 (with some changes in protocols)
* PCR amplification of pSB1A3, pSB1C3 and pSB1T3 (with some changes in protocols)
* PCR amplification of BioBrick parts using new primers
* PCR amplification of BioBrick parts using new primers
-
===[[Team:HokkaidoU_Japan/Notebook/August24|Tuesday, August 24, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August24|Tuesday, August 24]]===
* Electrophoresis of PCR products that had been amplified using new primers
* Electrophoresis of PCR products that had been amplified using new primers
* Examination of two ligation kits
* Examination of two ligation kits
Line 373: Line 373:
* Transformation of 1-3A (RFP reporter with chloramphenicol resistance) to evaluate the medium
* Transformation of 1-3A (RFP reporter with chloramphenicol resistance) to evaluate the medium
-
===[[Team:HokkaidoU_Japan/Notebook/August25|Wednesday, August 25, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August25|Wednesday, August 25]]===
* Estimation of the amount of 1-3A DNA that could transform and grow cells on the chloramphenicol medium
* Estimation of the amount of 1-3A DNA that could transform and grow cells on the chloramphenicol medium
* Ethanol precipitation to condense the pSB1C3 DNA solution, and its digestion and ligation
* Ethanol precipitation to condense the pSB1C3 DNA solution, and its digestion and ligation
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* Digestion of PCR products that had been amplified by using new primers
* Digestion of PCR products that had been amplified by using new primers
-
===[[Team:HokkaidoU_Japan/Notebook/August26|Thursday, August 26, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August26|Thursday, August 26]]===
* Electriphoresis to check whether ligation had been successful
* Electriphoresis to check whether ligation had been successful
* Digestion of PCR products that had been amplified using new primers (with reconstruction of reaction system)
* Digestion of PCR products that had been amplified using new primers (with reconstruction of reaction system)
-
===[[Team:HokkaidoU_Japan/Notebook/August27|Friday, August 27, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August27|Friday, August 27]]===
* Ligation of 1-1A (RFP reporter device) into pSB1C3 and its transformation
* Ligation of 1-1A (RFP reporter device) into pSB1C3 and its transformation
* Retry of 3 piece ligation which was done on August 19th and 20th
* Retry of 3 piece ligation which was done on August 19th and 20th
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----
----
-
===[[Team:HokkaidoU_Japan/Notebook/August30|Monday, August 30, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August30|Monday, August 30]]===
* Measurement of restriction enzyme activity using highly purified pUC119
* Measurement of restriction enzyme activity using highly purified pUC119
* Digestion of PCR products that had been amplified using new primers
* Digestion of PCR products that had been amplified using new primers
-
===[[Team:HokkaidoU_Japan/Notebook/August31|Tuesday, August 31, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/August31|Tuesday, August 31]]===
* Gel extraction, ligation and transformation of pUC119
* Gel extraction, ligation and transformation of pUC119
-
===[[Team:HokkaidoU_Japan/Notebook/September1|Wednesday, September 1, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/September1|Wednesday, September 1]]===
* PCR amplification of 1-5A (RFP reporter)
* PCR amplification of 1-5A (RFP reporter)
-
===[[Team:HokkaidoU_Japan/Notebook/September2|Thursday, September 2, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/September2|Thursday, September 2]]===
* Digestion, ligation and Transformation of RFP reporter with pSB1C3 or pUC119
* Digestion, ligation and Transformation of RFP reporter with pSB1C3 or pUC119
-
===[[Team:HokkaidoU_Japan/Notebook/September3|Friday, September 3, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/September3|Friday, September 3]]===
* Colony PCR of ''E. coli''  transformed on previous day
* Colony PCR of ''E. coli''  transformed on previous day
* PCR amplification of 1-3A (RFP reporter)
* PCR amplification of 1-3A (RFP reporter)
-
===[[Team:HokkaidoU_Japan/Notebook/September4|Saturday, September 4, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/September4|Saturday, September 4]]===
* PCR amplification of 1-5A (RFP reporter)
* PCR amplification of 1-5A (RFP reporter)
* Estimation of the amount of 1-3A PCR product
* Estimation of the amount of 1-3A PCR product
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----
----
-
===[[Team:HokkaidoU_Japan/Notebook/September6|Monday, September 6, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/September6|Monday, September 6]]===
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===[[Team:HokkaidoU_Japan/Notebook/September7|Tuesday, September 7, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September7|Tuesday, September 7]]===
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===[[Team:HokkaidoU_Japan/Notebook/September8|Wednesday, September 8, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September8|Wednesday, September 8]]===
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===[[Team:HokkaidoU_Japan/Notebook/September9|Thursday, September 9, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September9|Thursday, September 9]]===
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===[[Team:HokkaidoU_Japan/Notebook/September10|Friday, September 10, 2010]]===
+
===[[Team:HokkaidoU_Japan/Notebook/September10|Friday, September 10]]===
----
----
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===[[Team:HokkaidoU_Japan/Notebook/September13|Monday, September 13, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September13|Monday, September 13]]===
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===[[Team:HokkaidoU_Japan/Notebook/September14|Tuesday, September 14, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September14|Tuesday, September 14]]===
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===[[Team:HokkaidoU_Japan/Notebook/September15|Wednesday, September 15, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September15|Wednesday, September 15]]===
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===[[Team:HokkaidoU_Japan/Notebook/September16|Thursday, September 16, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September16|Thursday, September 16]]===
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===[[Team:HokkaidoU_Japan/Notebook/September17|Friday, September 17, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September17|Friday, September 17]]===
----
----
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===[[Team:HokkaidoU_Japan/Notebook/September20|Monday, September 20, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September20|Monday, September 20]]===
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===[[Team:HokkaidoU_Japan/Notebook/September21|Tuesday, September 21, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September21|Tuesday, September 21]]===
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===[[Team:HokkaidoU_Japan/Notebook/September22|Wednesday, September 22, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September22|Wednesday, September 22]]===
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===[[Team:HokkaidoU_Japan/Notebook/September23|Thursday, September 23, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September23|Thursday, September 23]]===
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===[[Team:HokkaidoU_Japan/Notebook/September24|Friday, September 24, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September24|Friday, September 24]]===
----
----
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===[[Team:HokkaidoU_Japan/Notebook/September27|Monday, September 27, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September27|Monday, September 27]]===
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===[[Team:HokkaidoU_Japan/Notebook/September28|Tuesday, September 28, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September28|Tuesday, September 28]]===
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===[[Team:HokkaidoU_Japan/Notebook/September29|Wednesday, September 29, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September29|Wednesday, September 29]]===
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===[[Team:HokkaidoU_Japan/Notebook/September30|Thursday, September 30, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/September30|Thursday, September 30]]===
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===[[Team:HokkaidoU_Japan/Notebook/October1|Friday, October 1, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October1|Friday, October 1]]===
----
----
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===[[Team:HokkaidoU_Japan/Notebook/October4|Monday, October 4, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October4|Monday, October 4]]===
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===[[Team:HokkaidoU_Japan/Notebook/October5|Tuesday, October 5, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October5|Tuesday, October 5]]===
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===[[Team:HokkaidoU_Japan/Notebook/October6|Wednesday, October 6, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October6|Wednesday, October 6]]===
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===[[Team:HokkaidoU_Japan/Notebook/October7|Thursday, October 7, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October7|Thursday, October 7]]===
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===[[Team:HokkaidoU_Japan/Notebook/October8|Friday, October 8, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October8|Friday, October 8]]===
----
----
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===[[Team:HokkaidoU_Japan/Notebook/October11|Monday, October 11, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October11|Monday, October 11]]===
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===[[Team:HokkaidoU_Japan/Notebook/October12|Tuesday, October 12, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October12|Tuesday, October 12]]===
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===[[Team:HokkaidoU_Japan/Notebook/October13|Wednesday, October 13, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October13|Wednesday, October 13]]===
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===[[Team:HokkaidoU_Japan/Notebook/October14|Thursday, October 14, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October14|Thursday, October 14]]===
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===[[Team:HokkaidoU_Japan/Notebook/October15|Friday, October 15, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October15|Friday, October 15]]===
----
----
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===[[Team:HokkaidoU_Japan/Notebook/October18|Monday, October 18, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October18|Monday, October 18]]===
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===[[Team:HokkaidoU_Japan/Notebook/October19|Tuesday, October 19, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October19|Tuesday, October 19]]===
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===[[Team:HokkaidoU_Japan/Notebook/October20|Wednesday, October 20, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October20|Wednesday, October 20]]===
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===[[Team:HokkaidoU_Japan/Notebook/October21|Thursday, October 21, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October21|Thursday, October 21]]===
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===[[Team:HokkaidoU_Japan/Notebook/October22|Friday, October 22, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October22|Friday, October 22]]===
----
----
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===[[Team:HokkaidoU_Japan/Notebook/October25|Monday, October 25, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October25|Monday, October 25]]===
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===[[Team:HokkaidoU_Japan/Notebook/October26|Tuesday, October 26, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October26|Tuesday, October 26]]===
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===[[Team:HokkaidoU_Japan/Notebook/October27|Wednesday, October 27, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October27|Wednesday, October 27]]===
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===[[Team:HokkaidoU_Japan/Notebook/October28|Thursday, October 28, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October28|Thursday, October 28]]===
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===[[Team:HokkaidoU_Japan/Notebook/October29|Friday, October 29, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/October29|Friday, October 29]]===
----
----
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===[[Team:HokkaidoU_Japan/Notebook/November1|Monday, November 1, 2010]]===
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===[[Team:HokkaidoU_Japan/Notebook/November1|Monday, November 1]]===
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===[[Team:HokkaidoU_Japan/Notebook/November29|Monday, November 29]]===
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===[[Team:HokkaidoU_Japan/Notebook/November30|Tuesday, November 30]]===

Revision as of 05:23, 11 September 2010


Calendar

July
SUN MON TUE WED THU FRI SAT
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
August
SUN MON TUE WED THU FRI SAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September
SUN MON TUE WED THU FRI SAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October
SUN MON TUE WED THU FRI SAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
November
SUN MON TUE WED THU FRI SAT
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
 

Abstract

Monday, July 12

  • Our first experiment, transformation of BioBrick devices.

Wednesday, July 21

  • Preparation of competent cells
  • Single colony isolation of E. coli which had been transformed on Monday, July 12

Monday, July 26

  • Cultivation of transformed E. coli for the next day's miniprep

Tuesday, July 27

  • Miniprep

Monday, August 2

  • Restriction enzyme digestion of plasmids which had been purified by miniprep
  • Electrophoresis assay

Tuesday, August 3

Wednesday, August 4

Thursday, August 5

Friday, August 6

Monday, August 9

  • Initial amplification of the BioBrick parts (BBa_I14032 "2-11P", BBa_F2621 "2-21H", BBa_K098995 "3-1E" and BBa_E1010 "1-18F") that are used in the construction of concentration-sensitive E. coli and heat-sensitive E. coli
  • These are preliminary experiments before starting the main project

Tuesday, August 10

  • PCR amplification of the other parts (BBa_F1610 "2-24G", BBa_B0034 "1-2M", BBa_B0015 "1-23L" and BBa_K098995 "3-1E") and electrophoresis to confirm

Wednesday, August 11

  • Preparation for the next day's miniprep
  • Estimation of enzyme activity
  • Preparation of the glycerol stocks

Thursday, August 12

  • Miniprep (1-18F, 2-21H and 2-11P)
  • Electrophoresis of the DNA which had been amplified via PCR and miniprep

Friday, August 13

  • PCR amplification of the DNA that we couldn't obtain via miniprep previous day(1-18F)

Monday, August 16

  • Measurement of restriction enzyme activity
  • Restriction enzyme digestion of the parts(3-1E, 1-2M, 1-18F and 1-23L) and the vector(pSB1C3)

Tuesday, August 17

  • Purification of the DNA solutions via gel extraction
  • Preparation of agar medium containing 35 ug/mL chloramphenicol

Wednesday, August 18

  • Digestion and gel extraction of 1-2M (retry)
  • 3 piece ligation of 1-18F, 1-23L and pSB1C3
  • Transformation

Thursday, August 19

  • 3 piece ligation of 3-1E (heat sensor), 1-2M (ribosome binding site) and pSB1C3 (vector)
  • Ligation that uses only vector pSB1C3 to estimate its efficiency

Friday, August 20

  • PCR amplification of pSB1A3, pSB1C3 and pSB1T3

Monday, August 23

  • PCR amplification of pSB1A3, pSB1C3 and pSB1T3 (with some changes in protocols)
  • PCR amplification of BioBrick parts using new primers

Tuesday, August 24

  • Electrophoresis of PCR products that had been amplified using new primers
  • Examination of two ligation kits
  • Electrophoresis assay of miscellaneous DNA solutions
  • Transformation of 1-3A (RFP reporter with chloramphenicol resistance) to evaluate the medium

Wednesday, August 25

  • Estimation of the amount of 1-3A DNA that could transform and grow cells on the chloramphenicol medium
  • Ethanol precipitation to condense the pSB1C3 DNA solution, and its digestion and ligation 
Ligationはお互いそれともパーツと？
ベクター同士．ご自分の記録をご確認ください．
  • Digestion of PCR products that had been amplified by using new primers

Thursday, August 26

  • Electriphoresis to check whether ligation had been successful
  • Digestion of PCR products that had been amplified using new primers (with reconstruction of reaction system)

Friday, August 27

  • Ligation of 1-1A (RFP reporter device) into pSB1C3 and its transformation
  • Retry of 3 piece ligation which was done on August 19th and 20th
  • Ligation of vectors to each other

Monday, August 30

  • Measurement of restriction enzyme activity using highly purified pUC119
  • Digestion of PCR products that had been amplified using new primers

Tuesday, August 31

  • Gel extraction, ligation and transformation of pUC119

Wednesday, September 1

  • PCR amplification of 1-5A (RFP reporter)

Thursday, September 2

  • Digestion, ligation and Transformation of RFP reporter with pSB1C3 or pUC119

Friday, September 3

  • Colony PCR of E. coli transformed on previous day
  • PCR amplification of 1-3A (RFP reporter)

Saturday, September 4

  • PCR amplification of 1-5A (RFP reporter)
  • Estimation of the amount of 1-3A PCR product

Monday, September 6

Tuesday, September 7

Wednesday, September 8

Thursday, September 9

Friday, September 10

Monday, September 13

Tuesday, September 14

Wednesday, September 15

Thursday, September 16

Friday, September 17

Monday, September 20

Tuesday, September 21

Wednesday, September 22

Thursday, September 23

Friday, September 24

Monday, September 27

Tuesday, September 28

Wednesday, September 29

Thursday, September 30

Friday, October 1

Monday, October 4

Tuesday, October 5

Wednesday, October 6

Thursday, October 7

Friday, October 8

Monday, October 11

Tuesday, October 12

Wednesday, October 13

Thursday, October 14

Friday, October 15

Monday, October 18

Tuesday, October 19

Wednesday, October 20

Thursday, October 21

Friday, October 22

Monday, October 25

Tuesday, October 26

Wednesday, October 27

Thursday, October 28

Friday, October 29

Monday, November 1

Tuesday, November 2

Wednesday, November 3

Thursday, November 4

Friday, November 5

Monday, November 8

Tuesday, November 9

Wednesday, November 10

Thursday, November 11

Friday, November 12

Monday, November 15

Tuesday, November 16

Wednesday, November 17

Thursday, November 18

Friday, November 19

Monday, November 22

Tuesday, November 23

Wednesday, November 24

Thursday, November 25

Friday, November 26

Monday, November 29

===Tuesday, November 30===
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/NotebookTest"