Team:UNIPV-Pavia/Calendar/July

From 2010.igem.org

(Difference between revisions)
Line 51: Line 51:
<tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 2</b></th></tr>
<tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 2</b></th></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 5th|July, 5th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 5th|July, 5th]]</td><td align="left">
-
21° Bio-Lab -
+
21° Bio-Lab - LB agar plates prepared.
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 6th|July, 6th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 6th|July, 6th]]</td><td align="left">
Line 57: Line 57:
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 7th|July, 7th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 7th|July, 7th]]</td><td align="left">
-
23° Bio-Lab - Lorenzo, Susanna Z., Sara, Nicolò.<br>
+
23° Bio-Lab - <br>
11° Meeting - Updating about Bio-Lab activity.
11° Meeting - Updating about Bio-Lab activity.
</td></tr>
</td></tr>
Line 71: Line 71:
25° Bio-Lab - All plates showed red colonies, so we calculated the efficiency of the strains.</td></tr>
25° Bio-Lab - All plates showed red colonies, so we calculated the efficiency of the strains.</td></tr>
<tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 3</b></th></tr>
<tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 3</b></th></tr>
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 12th|July, 12th]]</td><td align="left">26° Bio-Lab - </td></tr>
+
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 12th|July, 12th]]</td><td align="left">
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 13th|July, 13th]]</td><td align="left">27° Bio-Lab - </td></tr>
+
26° Bio-Lab -  
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 14th|July, 14th]]</td><td align="left">28° Bio-Lab - </td></tr>
+
</td></tr>
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 15th|July, 15th]]</td><td align="left">29° Bio-Lab - </td></tr>
+
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 13th|July, 13th]]</td><td align="left">
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 16th|July, 16th]]</td><td align="left">30° Bio-Lab - </td></tr>
+
27° Bio-Lab -  
 +
</td></tr>
 +
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 14th|July, 14th]]</td><td align="left">
 +
28° Bio-Lab -  
 +
</td></tr>
 +
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 15th|July, 15th]]</td><td align="left">
 +
29° Bio-Lab -  
 +
</td></tr>
 +
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 16th|July, 16th]]</td><td align="left">
 +
30° Bio-Lab -  
 +
</td></tr>
<tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 4</b></th></tr>
<tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 4</b></th></tr>
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 19th|July, 19th]]</td><td align="left">31° Bio-Lab - Susy Z. Nicolò, Federica, Matteo M.</td></tr>
+
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 19th|July, 19th]]</td><td align="left">
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 20th|July, 20th]]</td><td align="left">32° Bio-Lab - Susy Z., Nicolò, Federica, Riccardo.</td></tr>
+
31° Bio-Lab -  
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 21st|July, 21st]]</td><td align="left">33° Bio-Lab - Susy Z, Nicolò, Sara, Alessandro</td></tr>
+
</td></tr>
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 22nd|July, 22nd]]</td><td align="left">34° Bio-Lab - Susy Z, Nicolò, Federica, Riccardo, Giacomo.</td></tr>
+
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 20th|July, 20th]]</td><td align="left">
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 23rd|July, 23rd]]</td><td align="left">35° Bio-Lab - Susy Z. Nicolò, Federica, Alessandro, Giacomo</td></tr>
+
32° Bio-Lab -  
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 24th|July, 24th]]</td><td align="left">36° Bio-Lab - </td></tr>
+
</td></tr>
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 25th|July, 25th]]</td><td align="left">37° Bio-Lab - </td></tr>
+
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 21st|July, 21st]]</td><td align="left">
 +
33° Bio-Lab -
 +
</td></tr>
 +
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 22nd|July, 22nd]]</td><td align="left">
 +
34° Bio-Lab -  
 +
</td></tr>
 +
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 23rd|July, 23rd]]</td><td align="left">
 +
35° Bio-Lab -  
 +
</td></tr>
 +
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 24th|July, 24th]]</td><td align="left">
 +
36° Bio-Lab -  
 +
</td></tr>
 +
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 25th|July, 25th]]</td><td align="left">
 +
37° Bio-Lab -  
 +
</td></tr>
<tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 5</b></th></tr>
<tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 5</b></th></tr>
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 26th|July, 26th]]</td><td align="left">38° Bio-Lab - <br> 12° Meeting - Updating about Bio-Lab activity and organization of our trip to Boston! </td></tr>
+
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 26th|July, 26th]]</td><td align="left">
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 27th|July, 27th]]</td><td align="left">39° Bio-Lab - </td></tr>
+
38° Bio-Lab - <br>  
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 28th|July, 28th]]</td><td align="left">40° Bio-Lab - </td></tr>
+
12° Meeting - Updating about Bio-Lab activity and organization of our trip to Boston!  
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 29th|July, 29th]]</td><td align="left">41° Bio-Lab - </td></tr>
+
</td></tr>
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 30th|July, 30th]]</td><td align="left">42° Bio-Lab - </td></tr>
+
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 27th|July, 27th]]</td><td align="left">
-
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 31th|July, 31th]]</td><td align="left">43° Bio-Lab - </td></tr>
+
39° Bio-Lab -  
 +
</td></tr>
 +
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 28th|July, 28th]]</td><td align="left">
 +
40° Bio-Lab -  
 +
</td></tr>
 +
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 29th|July, 29th]]</td><td align="left">
 +
41° Bio-Lab -  
 +
</td></tr>
 +
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 30th|July, 30th]]</td><td align="left">
 +
42° Bio-Lab -  
 +
</td></tr>
 +
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 31th|July, 31th]]</td><td align="left">
 +
43° Bio-Lab -  
 +
</td></tr>
</table>
</table>
</td>
</td>

Revision as of 08:49, 9 September 2010


JULY



Bio-Lab activity proceeds!

Select the day you are interested in to find out the details!

DATEACTIVITY
July, 1st

19° Bio-Lab - I7-3,4 and 5 and I8-3, 4 and 5 were prepared for TECAN test.
Of the plates incubated yesterday, a colony was picked and grown in 1ml LB (+ antibiotic) for 6 hours at 37°C 220 rpm. Other parts were received from Anderson Lab.

July, 2nd

20° Bio-Lab - TECAN test provided encouraging results, showing that I7-3, I7-5, I8-4 and I8-5 produced GFP.
Other culture (I7-1, I7-2, I7-4, I8-1, I8-2, I8-3) did not produce GFP, so they were thrown away. A furhter screening were performed on I7-3, I7-5, I8-4, I8-5 and this time all the clones are OK . Inoculum of <partinfo>BBa_J72007</partinfo> and <partinfo>BBa_J72013</partinfo> from LB agar plates.A glycerol stock was prepared for each culture.
Inoculum of <partinfo>BBa_J72008</partinfo> from LB agar plate grown at 30°C 220 rpm overnight.

JULY: WEEK 2
July, 5th

21° Bio-Lab - LB agar plates prepared.

July, 6th

22° Bio-Lab -

July, 7th

23° Bio-Lab -
11° Meeting - Updating about Bio-Lab activity.

July, 8th

24° Bio-Lab - Transformation of <partinfo>BBa_J23118</partinfo> in our home-made competent strains

  • MG1655
  • BW25141
  • BW25142
  • BW53474
  • DH5alpha (as control)
to check transformation efficiency.
July, 9th 25° Bio-Lab - All plates showed red colonies, so we calculated the efficiency of the strains.
JULY: WEEK 3
July, 12th

26° Bio-Lab -

July, 13th

27° Bio-Lab -

July, 14th

28° Bio-Lab -

July, 15th

29° Bio-Lab -

July, 16th

30° Bio-Lab -

JULY: WEEK 4
July, 19th

31° Bio-Lab -

July, 20th

32° Bio-Lab -

July, 21st

33° Bio-Lab -

July, 22nd

34° Bio-Lab -

July, 23rd

35° Bio-Lab -

July, 24th

36° Bio-Lab -

July, 25th

37° Bio-Lab -

JULY: WEEK 5
July, 26th

38° Bio-Lab -
12° Meeting - Updating about Bio-Lab activity and organization of our trip to Boston!

July, 27th

39° Bio-Lab -

July, 28th

40° Bio-Lab -

July, 29th

41° Bio-Lab -

July, 30th

42° Bio-Lab -

July, 31th

43° Bio-Lab -