Team:UC Davis/protocols/transformation.html
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<li><a href="https://2010.igem.org/Team:UC_Davis/protocols/luriabroth.html" class="help">luria broth</a></li> | <li><a href="https://2010.igem.org/Team:UC_Davis/protocols/luriabroth.html" class="help">luria broth</a></li> | ||
<li><a href="https://2010.igem.org/Team:UC_Davis/protocols/agarplates.html" class="help">nutrient agar plates</a></li> | <li><a href="https://2010.igem.org/Team:UC_Davis/protocols/agarplates.html" class="help">nutrient agar plates</a></li> | ||
+ | <li>Sterilized glass beads <li> | ||
</ul><p> | </ul><p> | ||
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<li>Incubate at 37°C for 1 hour in a shaker. </li> | <li>Incubate at 37°C for 1 hour in a shaker. </li> | ||
<li>Plate 200μL of each sample on <a href="https://2010.igem.org/Team:UC_Davis/protocols/luriabroth.html" class="help">nutrient agar plates.</a> (Be sure to choose the plates with the antibiotic that your E. Coli is resistant to.) </li> | <li>Plate 200μL of each sample on <a href="https://2010.igem.org/Team:UC_Davis/protocols/luriabroth.html" class="help">nutrient agar plates.</a> (Be sure to choose the plates with the antibiotic that your E. Coli is resistant to.) </li> | ||
+ | <li>Spread the sample evenly on the plate by plating with sterilized glass beads. Empty the plate of glass beads afterwards. </li> | ||
<li>Incubate overnight at 37°C. </li> | <li>Incubate overnight at 37°C. </li> | ||
</ul> | </ul> |
Revision as of 00:16, 8 September 2010
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