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Team:Tsinghua/Notebook/12 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Module I, DT and Fan's part: == Use the K-up and K-do to detect the P+K+C. Meanwhile use the digestion protocol to ensure the result. Double digestion system: P+E+M: H2O ...) |
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P+E+M② is positive. It seems that PKC 1-6 are positive. But it still remains unclear whether the PCR bands are so weak and why the digestion result seemed negative. | P+E+M② is positive. It seems that PKC 1-6 are positive. But it still remains unclear whether the PCR bands are so weak and why the digestion result seemed negative. | ||
Revision as of 10:16, 3 September 2010
Module I, DT and Fan's part:
Use the K-up and K-do to detect the P+K+C. Meanwhile use the digestion protocol to ensure the result.
Double digestion system:
P+E+M:
H2O 5.5-10.5μl buffer BamHI 2μl P+E+M 5-10μl SalI 1.5μl BamHI 1μl Total 20μl
P+K+C:
H2O 0-10.5μl buffer BamHI 2μl P+K+C 5-16μl KpnI 1.5μl BamHI 1μl Total 20μl
Run a gel. Order: P+E+M 1-6 double digestion, Marker, P+K+C 1-6 double digestion, P+K+C PCR.
result
P+E+M② is positive. It seems that PKC 1-6 are positive. But it still remains unclear whether the PCR bands are so weak and why the digestion result seemed negative.
