Team:Tsinghua/Notebook/13 July 2010

From 2010.igem.org

(Difference between revisions)
(New page: == Module I, DT and Fan's part: == PCR again with 35 cycles. Run a gel, cut and gel extract. We have improved our protocol for ligation, that is, ligate the first two and last two fragmen...)
(Module I, DT and Fan's part:)
 
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== Module I, DT and Fan's part: ==
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== Module I, group 2(b) ==
PCR again with 35 cycles. Run a gel, cut and gel extract. We have improved our protocol for ligation, that is, ligate the first two and last two fragments first, if the concentrate is low, go through a PCR and link them.
PCR again with 35 cycles. Run a gel, cut and gel extract. We have improved our protocol for ligation, that is, ligate the first two and last two fragments first, if the concentrate is low, go through a PCR and link them.

Latest revision as of 06:50, 25 October 2010

Module I, group 2(b)

PCR again with 35 cycles. Run a gel, cut and gel extract. We have improved our protocol for ligation, that is, ligate the first two and last two fragments first, if the concentrate is low, go through a PCR and link them.