Team:Tsinghua/Notebook/13 July 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Module I, DT and Fan's part: == PCR again with 35 cycles. Run a gel, cut and gel extract. We have improved our protocol for ligation, that is, ligate the first two and last two fragmen...) |
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PCR again with 35 cycles. Run a gel, cut and gel extract. We have improved our protocol for ligation, that is, ligate the first two and last two fragments first, if the concentrate is low, go through a PCR and link them. | PCR again with 35 cycles. Run a gel, cut and gel extract. We have improved our protocol for ligation, that is, ligate the first two and last two fragments first, if the concentrate is low, go through a PCR and link them. |
Latest revision as of 06:50, 25 October 2010
Module I, group 2(b)
PCR again with 35 cycles. Run a gel, cut and gel extract. We have improved our protocol for ligation, that is, ligate the first two and last two fragments first, if the concentrate is low, go through a PCR and link them.