Team:LMU-Munich/Schedule

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PCR and mutagenesis of the digestion sites

Primer table : Primer sequences, intern numeration, Tm

PCR1a: replication of Pdu D with mutagenesis [ ]

Primer used: 1P1D, mpduD rev expected product size: bp

PCR1b: replication of Pdu D with mutagenesis [ ]

Primer used: mpduD fwd, 3P2D expected product size: bp

PCR2: Joining PCR of PduD [ ]

Primer used: 1P1D, 3P2D expected product size: bp

PCR3a: replication of Pdu C with mutagenesis [ ]

Primer used: 2P1C, mpduC rev expected product size: bp

PCR3b: replication of Pdu C with mutagenesis [ ]

Primer used: mpduC fwd, 4P2C expected product size: bp

PCR4: Joining PCR of Pdu C [ ]

Primer used: 2P1C, 4P2C expected product size: bp

PCR5: replication of AurF to be joined with Pdu D [ ]

Primer used: 5P3AD, 9P4A expected product size: bp

PCR6a: replication of LctO with mutagenesis [ ]

Primer used: 8P3LC, m1lctO rev expected product size: bp

PCR6b: replication of LctO with mutagenesis [ ]

Primer used: m1lctO fwd, 10P4L expected product size: bp

PCR7: Joining PCR of LctO [ ]

Primer used: 8P3LC, 10P4L expected product size: bp

PCR8a: replication of LctO with mutagenesis [ ]

Primer used: 8P3LC, m2lctO rev expected product size: bp

PCR8b: replication of LctO with mutagenesis [ ]

Primer used: m2lctO fwd, 10P4L expected product size: bp

PCR9: Joining PCR of LctO [ ]

Primer used: 8P3LC, 10P4L expected product size: bp

PCR10: Joining PCR of AurF and PduD [ ]

Primer used: 1P1D, 9P4A expected product size: bp

PCR11: Joining PCR of LctO and PduC [ ]

Primer used: 2P1C, 10P4L expected product size: bp

PCR12a: replication of the Pdu-Operon with mutagenesis [ ]

Primer used: pduA fwd, mpduA rev expected product size: bp

PCR12b: replication of the Pdu-Operon with mutagenesis [ ]

Primer used: mpduA fwd,pduU rev expected product size: bp

PCR13: Joining PCR of the Pdu-Operon [ ]

Primer used: pduA fwd,pduU rev expected product size: bp

PCR14a: replication of the Pdu-Operon with mutagenesis [ ]

Primer used: pduA fwd,mpduB rev expected product size: bp

PCR14b: replication of the Pdu-Operon with mutagenesis [ ]

Primer used: mpduB fwd,pduU rev expected product size: bp

PCR15: Joining PCR of the Pdu-Operon [ ]

Primer used:pduA fwd,pduU rev expected product size: bp

PCR16a: replication of the Pdu-Operon with mutagenesis [ ]

Primer used:pduA fwd,mpduJ rev expected product size: bp

PCR16b: replication of the Pdu-Operon with mutagenesis [ ]

Primer used:mpduJ fwd,pduU rev expected product size: bp

PCR17: Joining PCR of the Pdu-Operon [ ]

Primer used:pduA fwd,pduU rev expected product size: bp

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Team:LMU-Munich/Schedule

From 2010.igem.org

Revision as of 15:00, 5 October 2010

Contents

PCR and mutagenesis of the digestion sites

In Vitro testing of the chemical kinetics with the enzymes

Transforming the construct in bacteria

Testing the system