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- | {{UT-Tokyo_HEADER}}
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- | <div id="main_all">
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- | = '''Sudoku''' =
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- | [https://2010.igem.org/Team:UT-Tokyo/Sudoku_abstract Abstract]
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- | [https://2010.igem.org/Team:UT-Tokyo/Sudoku_construct Construct]
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- | [https://2010.igem.org/Team:UT-Tokyo/Sudoku_experiment Experiment]
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- | Note
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- | [https://2010.igem.org/Team:UT-Tokyo/Sudoku_result Result]
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- | == '''Note''' ==
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- | == Pre-experiment ==
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- | === Make construct ===
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- | ==== 7/1 ====
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- | '''Transformation'''
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- | 2-3 plasmid (6/30 ligation)
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- | < 6/30 transformation → fail >
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- | '''Inoculation'''
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- | 11 plasmid (6/30 thaw, transformation)
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- | '''Enzyme cut'''
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- | 2, 3, 5, 7 plasmid (6/25 miniprep)
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- | 2 : 53.9 ng/uL → about 15 uL (shortage) / H / SP (2 uL)
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- | 3 : 124.2 ng/uL → 8 uL / M / XP (2 uL)
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- | 5 : 81.3 ng/uL → 12.3 uL / H / SP (2 uL)
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- | 7 : 85.4 ng/uL → 11.7 uL / H / SP (2 uL)
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- | '''Gel extract'''
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- | 2, 3, 5, 7 plasmid (7/1 enzyme cut)
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- | Label : 100701 数X (2,3,5,9) ゲル抽出
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- | ==== 7/2 ====
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- | '''Miniprep'''
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- | 2-3, 11 plasmid (7/1 inoculation)
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- | 2-3 → 105 ng/uL
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- | 11 (1st) → 191.3 ng/uL
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- | 11 (2nd) → 49.5 ng/uL
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- | '''Enzyme cut'''
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- | 2-3, 11 plasmid (7/2 miniprep)
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- | 2-3 : 9.5 uL / H / ES (1.5 uL)
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- | 11 (1st) : 5.2 uL / H / ES (1.5 uL) → Judged failure by cut check → miniprep again
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- | 11 (2nd) : 20.2 uL / H / ES (1.5 uL)
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- | ==== 7/3 ====
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- | '''Gel extract'''
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- | 2-3, 11 plasmid (7/2 enzyme cut)
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- | Label : 100703 X ゲル抽出
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- | ==== 7/5 ====
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- | '''Ligation'''
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- | 2-3 (7/3 enzyme cut) & 4 / 11 (7/3 enzyme cut) & 7
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- | 2-3 : 37.5 ng/uL → 5 uL & 4 : 5.2 ng/uL → 5 uL
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- | 11 : 4.2 ng/uL → 7.5 uL & 7 : Unknown → 2.5 uL
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- | '''Transformation'''
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- | 2-3-4 plasmid (7/5 ligation) / 11-7 plasmid (7/5 ligation)
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- | === Check test ===
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- | == Main construct ==
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- | === Research of SP6 RNApol cDNA and F plasmid ===
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- | === Convert lox71(rev) to lox66(rev) ===
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- | === Make Hin(rev) ===
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- | === Revise MS2 gene ===
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- | === Make location(rev)-rbs(rev)-location(rev) ===
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- | </div>
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- | {{UT-Tokyo_FOOTER}}
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