Team:UC Davis

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Revision as of 21:46, 1 September 2010

Welcome to our page! Our team is comprised of 8 dedicated individuals: 6 undergraduates and 2 advisors. This will be the second year that iGEM @ UC Davis participates in the competition. We are hard at work and are looking forward towards the completion of our project. Stay tuned for the upcoming results!

Updates

8.25.10 We have successfully pieced numerous parts together. After a couple more ligations and sequencing to confirm the parts, we will be entering testing phase!

7.19.10 The team has had a run-in with some major issues cutting parts out. We have traced the source of the problem and have been getting more positive results ever since.

7.8.10 We have our model and simulation completed!

6.30.10 We have developed a workplan for physically assembling and testing our devices.

6.28.10 We have finalized our project and have extracted our parts.

Throughout evolutionary history, spatial pattern formation has played a vital role in developmental biology. This is seen clearly in nature throughout the eukaryotic domain; examples include coat patterns (think zebras) and body segmentation (differentiated stem cells). We want to bring this sort of spatial pattern creation to the prokaryotic world. Previous iGEM projects have created patterns that require a projection of some sort of image before the cells react. We are engineering a strain that will create a pattern with no input from outside the system except an inducer.

This genetic circuit allows us to create biological systems with spatially varying genetic expression profiles. This has applications in a variety of fields such as nanofabrication, tissue engineering, environmental engineering, and of course, synthetic biology.

Read more...

A good scientist always keeps a lab notebook at hand in order to keep track of what they do. This ensures that they have a written record of their data, allows others to retrace their steps, and most importantly of all, back up their research findings. Sift through our notebook pages to see how this project was built!

Overall Workplan

Get the big picture of what we are trying to build!

Assembly Workplan

Our parts didn't just come together magically. Learn about how we assembled our parts piece by piece! Also, stay tuned for possible protocols that may save you hours on your experiment.

Testing & Validation

Building our parts also include testing our parts and ensuring that they work, since we cannot see what is actually going on with the naked eye. Learn how we identified problems, validated our experiments, and how we overcame various issues.

Using mathematical modeling, we were able create a computer simulation of how our lawn of E. Coli will look like. The red plane in the center is the original stimulus that triggered the striping.

Read more...

We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

No synthetic biology team should go without considering the potential dangers that their project(s) may cause! Because science can be prone to error, we ensured that our project is safe on many different levels.

Read more...
Criteria

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