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	[[Media:General_DNA_Digest_Protocol.pdf|DNA Digest]]		[[Media:General_DNA_Digest_Protocol.pdf|DNA Digest]]
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		+	[[Media:_Digest_reagents_calculator.xls|Digest Reagents Calculator]]
	[[Media:Protocol_for_Running_a_Gel.pdf|Running a Gel]]		[[Media:Protocol_for_Running_a_Gel.pdf|Running a Gel]]

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Revision as of 01:43, 7 September 2010

Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Epifluorescence Microscope Usage - H.H. Dow

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

Culturing CGSC Strains

OD600 Cell Dilution Protocol

DNA Manipulation

Transformation-heat shock

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Heat shock

Transformation-electroporation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

MODIFIED Miniprep

• Miniprep

Nanodrop

DNA Digest

Digest Reagents Calculator

Running a Gel

DNA Purification

Gel extraction

Updated T4 DNA Ligase Protocol (Not quick ligase)

• Ligation
  • Ginko Bioworks Protocol
  • Jeremy Minty Protocol

Group-Specific Protocols

Oil Sands

Revised biofilm assay protocol

• biofilm assay protocol

General primer design

Detailed primer design colony PCR protocol

Pili

FimB Ligation

Quorum Sensing

Logic Table

Lsr Circuit Test Protocols

General Outline for Biobrick Manipulations