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Latest revision as of 01:44, 24 August 2010

Insert weekly-ish updates here :)

Throughout evolutionary history, spatial pattern formation has played a vital role in developmental biology. This is seen clearly in nature throughout the eukaryotic domain; examples include coat patterns (think zebras) and body segmentation (differentiated stem cells). We want to bring this sort of spatial pattern creation to the prokaryotic world. Previous iGEM projects have created patterns that require a projection of some sort of image before the cells react. We are engineering a strain that will create a pattern with no input from outside the system except an inducer.

This genetic circuit allows us to create biological systems with spatially varying genetic expression profiles. This has applications in a variety of fields such as nanofabrication, tissue engineering, environmental engineering, and of course, synthetic biology.

A good scientist always keeps a lab notebook at hand in order to keep track of what they do. This ensures that they have a written record of their data, allows others to retrace their steps, and most importantly of all, back up their research findings. Sift through our notebook pages to see how this project was built!

Get the big picture of what we are trying to build!

Our parts didn't just come together magically. Learn about how we assembled our parts piece by piece! Also, stay tuned for possible protocols that may save you hours on your experiment.

Building our parts also include testing our parts and ensuring that they work, since we cannot see what is actually going on with the naked eye. Learn how we identified problems, validated our experiments, and how we overcame various issues.

Using mathematical modeling, we were able create a computer simulation of how our lawn of E. Coli will look like. The red plane in the center is the original stimulus that triggered the striping.

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-We are still in the process of listing our sponsors, so if you do not see your company/name yet, just know that you will be acknowledged.
+Want to sponsor us?  Send an email to <a href="mailto:mtfacciotti@ucdavis.edu">mtfacciotti@ucdavis.edu</a> to discuss various ways you can help! :)
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